Frozen therapeutic dose and package

Abstract

A frozen therapeutic dose includes an amniotic material and is configured into a pack for easy administering of the dose to a treatment location. A frozen therapeutic dose may contain a concentration of live amniotic stem cells. A frozen therapeutic dose may be provided in a form, such as a multi-pack form, to enable a person to administer a dose to a treatment location without the need of traveling to a doctor's office or clinic. A frozen therapeutic dose package may be kept in a conventional freezer at −20° C., for example, for extended periods of time and a person may remove the package as needed for treatment. A frozen dose package or pack may contain a secondary material configured to mix with the frozen therapeutic dose. A secondary material may be configured within a single dose compartment with the frozen dose or within a separate compartment.

What is claimed is: 
     
         1 . A therapeutic dose package comprising:
 a) a first compartment comprising:
 i) a frozen therapeutic dose comprising:
 amnion material. 
 
   
     
     
         2 . The therapeutic dose package of  claim 1 , wherein the frozen therapeutic dose has a volume of no more than about 40 ml. The therapeutic dose package of  claim 1 , wherein the frozen therapeutic dose comprises a therapeutic composition comprising:
 i) acellular amniotic membrane particles;   ii) a carrier fluid comprising an acellular amniotic fluid;   
       wherein the acellular amniotic membrane particles consist essentially of micronized amniotic membrane; and 
       wherein the therapeutic composition is essentially free of any viable amniotic membrane cells or viable amniotic fluid cells. 
     
     
         3 . The therapeutic dose package of  claim 1 , wherein the frozen therapeutic dose comprises a plurality of amniotic stem cells. 
     
     
         4 . The therapeutic dose package of  claim 1 , wherein the frozen therapeutic dose comprises a plurality of non-amnion derived cells. 
     
     
         5 . The therapeutic dose package of  claim 1 , wherein the non-amnion derived cells comprise stromal vascular fraction cells. 
     
     
         6 . The therapeutic dose package of  claim 1 , wherein the non-amnion derived cells comprise stem cells. 
     
     
         7 . The therapeutic dose package of  claim 1 , wherein the frozen therapeutic dose comprises a plurality of live amniotic stem cells. 
     
     
         8 . The therapeutic dose package of  claim 1 , wherein the frozen therapeutic dose comprises a plurality of particles comprising micronized amniotic membrane particles. 
     
     
         9 . The therapeutic dose package of  claim 8 , wherein the frozen therapeutic dose comprises live amniotic stem cells. 
     
     
         10 . The therapeutic dose package of  claim 1 , wherein the frozen therapeutic dose comprises amniotic fluid. 
     
     
         11 . The therapeutic dose package of  claim 10 , wherein the frozen therapeutic dose comprises live amniotic stem cells. 
     
     
         12 . The therapeutic dose package of  claim 1 , wherein the frozen therapeutic dose comprises amnion derived materials selected from the group consisting of: cytokines, proteins, growth factors, and collagen. 
     
     
         13 . The therapeutic dose package of  claim 1 , wherein the frozen therapeutic dose comprises:
 a) a plurality of particles comprising micronized amniotic membrane particles; and   b) amniotic fluid.   
     
     
         14 . The therapeutic dose package of  claims 13 , wherein the frozen therapeutic dose comprises live amniotic stem cells. 
     
     
         15 . The therapeutic dose package of  claim 1 , wherein the frozen therapeutic dose is a solid. 
     
     
         16 . The therapeutic dose package of  claim 1 , wherein the frozen therapeutic dose further comprising a cryoprotectant. 
     
     
         17 . The therapeutic dose package of  claim 16 , wherein the cryoprotectant comprises DMSO. 
     
     
         18 . The therapeutic dose package of  claim 1 , wherein the buffer comprises a pH buffer. 
     
     
         19 . The therapeutic dose package of  claim 1 , wherein the frozen therapeutic dose further comprising a diluent. 
     
     
         20 . The therapeutic dose package of  claim 19 , wherein the diluent comprises a plasma containing diluent.