Cytomimetic formulations and methods of manufacturing the same

Abstract

A cytomimetic formulation is provided comprising at least two of: (a) a fermented truffle extract; (b) a plurality of hyaluronic acids of different molecular weight, ranging from 50 KDa up to 2000 KDa; (c) an olive leaf extract in a mineral-containing water; and (d) a fermented grape must. The formulations mimic the skin cytoplasmic environment and create optimal conditions for cellular growth and skin rejuvenation. Methods of use and processes for manufacturing thereof are also provided.

1 . A cytomimetic formulation comprising at least two of:
 (a) a fermented truffle extract;   (b) a plurality of hyaluronic acids of different molecular weight, ranging from 50 KDa up to 2000 KDa;   (c) an olive leaf extract in a mineral-containing water; and   (d) a fermented grape must.   
     
     
         2 . The cytomimetic formulation of  claim 1 , further comprising:
 (e) a carrier suitable for topical application to human skin.   
     
     
         3 . The cytomimetic formulation of  claim 1 , wherein when applied to human skin the formulation elicits or enhances at least one of
 (i) cell growth,   (ii) skin rejuvenation,   (iii) counteraction of one or more features of skin aging, and   (iv) skin tissue wound repair.   
     
     
         4 . The cytomimetic formulation of  claim 1 , wherein the formulation comprises fermented truffle extract and the fermented truffle extract is obtained by a process comprising the following steps:
 (a) homogenizing a truffle tuber in a physiological solution to form a homogenate;   (b) fermenting with one or more microorganisms the homogenate to form a fermentate;   (c) filtering the fermentate to remove particulate matters to form a filtered fermentate;   (e) drying filtered fermentate by lyophilization or spray drying so as to obtain a dry fermented truffle extract.   
     
     
         5 . The cytomimetic formulation of  claim 1 , wherein the truffle tuber is  Tuber magnatum preciosa.    
     
     
         6 . The cytomimetic formulation of  claim 4 , wherein the one or more microorganism comprises  Saccharomyces cerevisiae.    
     
     
         7 . The cytomimetic formulation of  claim 1 , wherein the formulation comprises the plurality of hyaluronic acids of different molecular weight, comprises hyaluronic acids of 1800 KDa, 800 KDa and 200 KDa. 
     
     
         8 . The cytomimetic formulation of  claim 7 , wherein the hyaluronic acids of 1800 KDa, 800 KDa and 200 KDa are present, each in a proportion of not less than 2% of the total weight of hyaluronic acids present. 
     
     
         9 . The cytomimetic formulation of  claim 1 , wherein the formulation comprises olive leaf extract and the olive leaf extract is obtained by a process comprising the steps of:
 (a) homogenizing fresh olive leaves in mineral-containing water solution to form a homogenate;   (b) extracting the homogenate for a predetermined period of time to form an extract; and   (c) filtering the extract to remove solid particulate matters, so as to obtain the olive leaf extract.   
     
     
         10 . The cytomimetic formulation of  claim 1  in which the mineral water is a thermal water obtained from Thurio Spring at Spezzano Thermal Baths, Calabria, Italy. 
     
     
         11 . The cytomimetic formulation of  claim 9 , wherein the solid/solvent ratio during extraction is 1 to 4 ratio in weight and the extraction time is 24-72 hours at 4° C. and pH 7. 
     
     
         12 . The cytomimetic formulation of  claim 1 , wherein the formulation comprises fermented grape must and the fermented grape must is obtained by a process comprising the steps of
 (a) obtaining freshly harvested grapes;   (b) recovering grape juice from the grapes by mechanical pressure;   (c) fermenting the grape juice to form a fermentate;   (d) filtering the fermentate to remove particulate so as to obtain a clear solution; and   (e) drying the clear solution by lyophilization or spray drying, so as to obtain dry fermented grape must.   
     
     
         13 . The cytomimetic formulation of  claim 12 , wherein the grapes are Aglianic grapes from the slopes of Mount Falernus, Italy. 
     
     
         14 . The cytomimetic formulation of  claim 12 , wherein the fermentation is performed at 10-15° C. for 24-48 hours. 
     
     
         15 . The cytomimetic formulation of  claim 1 , which is a cosmetic formulation. 
     
     
         16 . The cosmetic formulation of  claim 15 , wherein the cosmetic formulation comprises from 0.1 to 50% w/w of each of following:
 (a) a fermented truffle extract;   (b) a plurality of hyaluronic acids of different molecular weight, ranging from 50 KDa up to 2000 KDa;   (c) an olive leaf extract in a mineral-containing water; and   (d) a fermented grape must.   
     
     
         17 . A cytomimetic formulation as recited in  claim 1  further comprising at least one of:
 (a) an aqueous phase; 
 (b) an oil phase; 
 (c) one or more preservatives; and 
 (d) one or more fragrances. 
 
     
     
         18 . (canceled) 
     
     
         19 . The cosmetic formulation of  claim 17 , wherein the aqueous phase comprises at least one of:
 (i) water,   (ii) glycerin,   (iii) glyceryl polyacrylate,   (iv) acrylates copolymer,   (v) butylene glycol,   (vi) carbomer, and   (vii) xanthan gum.   
     
     
         20 . (canceled) 
     
     
         21 . The cosmetic formulation of  claim 17 , wherein the oil phase comprises at least one of:
 (i)  Olea europaea  fruit oil;   (ii) stearoxymethicone/dimethicone copolymer;   (iii) polymethylsilsesquioxane;   (iv) polyacrylate-13;   (v) di/trimethylol hexylactone crosspolymer;   (vi) polyisobutene;   (vii) cholesteryl nonanoate;   (viii) hydrogenated lecithin;   (ix) polysorbate 20;   (x) cholesteryl chloride;   (xi) sodium acrylates copolymer;   (xii) cholesteryl oleyl carbonate;   (xiii) silica; and   (xiv) methyl methacrylate crosspolymer.   
     
     
         22 - 25 . (canceled) 
     
     
         26 . The cytomimetic or cosmetic formulation of  claim 1 , which is a skincare formulation, a hair product, a scalp product, or a makeup formulation. 
     
     
         27 - 34 . (canceled)