Insecticidal cry toxins

Abstract

Insecticidal toxins derived from Bacillus thuringiensis, polynucleotides encoding such toxins, use of such toxins to control Coleopteran plant pests, and transgenic plants that produce, and are protected, by these toxins are described.

What is claimed is: 
     
         1 . A nucleic acid construct comprising a nucleic acid sequence encoding an insecticidal IRDIG protein. 
     
     
         2 . A nucleic acid construct comprising a nucleic acid sequence of  claim 1  chosen from the group consisting of:
 a. insecticidal IRDIG proteins having at least 80% sequence identity with any one of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 71, 73, 75, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, and 122; 
 b. insecticidal IRDIG proteins having at least 90% sequence identity with any one of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 71, 73, 75, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, and 122; 
 c. insecticidal IRDIG proteins having at least 95% sequence identity with any one of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 71, 73, 75, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, and 122; 
 d. insecticidal IRDIG proteins having at least 99% sequence identity with any one of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 71, 73, 75, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, and 122, and; 
 e. insecticidal IRDIG proteins having 100% sequence identity with any one of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 71, 73, 75, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, and 122. 
 
     
     
         3 . A composition comprising a formulated insecticidal IRDIG protein. 
     
     
         4 . A composition comprising a formulated insecticidal IRDIG protein in which the insecticidal IRDIG protein is chosen from the group consisting of:
 a. insecticidal IRDIG proteins having at least 80% sequence identity with any one of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 71, 73, 75, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, and 122;   b. insecticidal IRDIG proteins having at least 90% sequence identity with any one of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 71, 73, 75, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, and 122;   c. insecticidal IRDIG proteins having at least 95% sequence identity with any one of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 71, 73, 75, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, and 122;   d. insecticidal IRDIG proteins having at least 99% sequence identity with any one of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 71, 73, 75, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, and 122, and;   e. insecticidal IRDIG proteins having 100% sequence identity with any one of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 71, 73, 75, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, and 122.   
     
     
         5 . A plant or plant part comprising a nucleic acid sequence encoding an insecticidal IRDIG protein comprising a nucleic acid sequence of  claim 1 . 
     
     
         6 . The plant part of  claim 5  wherein the plant part is a seed. 
     
     
         7 . A plant or plant part comprising a polynucleotide comprising a sequence of  claim 2 . 
     
     
         8 . The plant part of  claim 7  wherein the plant part is a seed. 
     
     
         9 . A plant or plant part comprising an insecticidal IRDIG protein comprising a sequence of  claim 3 . 
     
     
         10 . The plant part of  claim 9  wherein the plant part is a seed. 
     
     
         11 . A plant or plant part comprising an insecticidal IRDIG protein comprising a sequence of  claim 4 . 
     
     
         12 . The plant or plant part of  claim 5 , wherein the insecticidal IRDIG protein has insecticidal activity against an insect selected from the group consisting of the order Coleoptera and Western Corn Rootworm. 
     
     
         13 . A plant or plant part of  claim 7  wherein the polypeptide has insecticidal activity against an insect selected from the group consisting of the order Coleoptera and Western Corn Rootworm. 
     
     
         14 . The plant or plant part of  claim 4 , wherein the polypeptide has insecticidal activity against an insect selected from the group consisting of the order Coleoptera and Western Corn Rootworm. 
     
     
         15 . A method for controlling susceptible insects comprising contacting said insect with an effective amount of an insecticidal IRDIG protein of  claim 3 . 
     
     
         16 . A method for controlling susceptible insects comprising contacting said insect with an effective amount of an insecticidal IRDIG protein of  claim 4 . 
     
     
         17 . The method of  claim 15  wherein the susceptible insect is Western Corn Rootworm 
     
     
         18 . The method of  claim 15  wherein the susceptible insect is Western Corn Rootworm. 
     
     
         19 . A method for producing a coleopteran-tolerant plant comprising breeding a non transgenic plant with a transgenic plant comprising a foreign DNA construct, capable of expressing an insecticidal IRDIG protein, stably incorporated into the genome of the plant and selecting progeny by analyzing for at least a portion of the foreign DNA construct emanating from the transgenic plant.