Magnetic cells for localizing delivery and tissue repair

Abstract

Normal or genetically modified cell(s) having magnetic nanoparticle(s) bound (affixed) to their surfaces and methods of delivery to target tissues, e.g. for treatment of disease and/or injury.

1 .- 27 . (canceled) 
     
     
         28 . A method of delivering a cell to a target tissue in an animal having a condition selected from selected from cirrhosis, heart failure, adenocarcinoma, carcinoma, lymphoma, skin wounds, stroke and spinal cord injury, said method comprising:
 administering a magnetic particle-comprising cell having at least one magnetic nanoparticle affixed to the surface of said cell to the animal; and   applying a magnetic field to said target tissue under conditions such that said magnetic particle-comprising cell is delivered to specific regions of said target tissue.   
     
     
         29 . The method of  claim 28 , wherein the magnetic particle is affixed to the cell surface by means of a cell-surface specific binding agent. 
     
     
         30 . The method of  claim 29 , wherein the cell-surface specific binding agent is an antibody. 
     
     
         31 . The method of  claim 28  wherein the magnetic particles have a mean diameter of no more than 500 nm. 
     
     
         32 . The method of  claim 30  wherein the magnetic particles have a mean diameter of no more than 200 nm. 
     
     
         33 . The method of  claim 28  wherein the magnetic particles comprise iron in any ferromagnetic form. 
     
     
         34 . The method of  claim 28  wherein the magnetic nanoparticles have a surface coating. 
     
     
         35 . The method of  claim 34  wherein the surface coating is chemically modified to allow the binding of an antibody, or antibody fragment, or protein or sugar fragment that binds to cells. 
     
     
         36 . The method of  claim 28  wherein the magnetic particle comprising cells are administered by injection or infusion or surface application. 
     
     
         37 . The method of  claim 28  wherein the animal has cirrhosis and hepatocytes are delivered to a liver, wherein the animal has heart failure and cardiac myocytes are delivered to a heart of the animal, wherein the animal has adenocarcinoma, carcinoma, or lymphoma and toxin-containing cells are delivered to a tumor of the animal, wherein the animal has a skin wound and fibroblasts and epithelial cells are delivered to the skin of the animal; wherein the animal has a stroke and stem cells are delivered to a brain of the animal, or wherein the animal has a spinal cord injury and stem cells, neurons, or immune cells are delivered to a spinal cord of the animal. 
     
     
         38 . The method of  claim 28  wherein the animal is a mammal. 
     
     
         39 . The method of  claim 38  wherein the mammal is a human.