Polypeptides having protease activity and polynucleotides encoding same

Abstract

The present invention relates to isolated polypeptides having protease activity, and polynucleotides encoding the polypeptides. The invention further relates to the use of such polypeptides in detergent and/or in cleaning processes. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides.

1 . A recombinant host cell comprising a nucleic acid construct or expression vector comprising a polynucleotide encoding a polypeptide having protease activity, wherein
 the polypeptide has at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 2; at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 4; or at least 89% sequence identity to the mature polypeptide of SEQ ID NO: 6; and   the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in the recombinant host cell.   
     
     
         2 . The recombinant host cell of  claim 1 , wherein the polypeptide has at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2; the mature polypeptide of SEQ ID NO: 4; or the mature polypeptide of SEQ ID NO: 6. 
     
     
         3 . The recombinant host cell of  claim 1 , wherein the polypeptide has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2; the mature polypeptide of SEQ ID NO: 4; or the mature polypeptide of SEQ ID NO: 6. 
     
     
         4 . The recombinant host cell of  claim 1 , wherein the polypeptide encoded by a polynucleotide having at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1; a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 3; or a polynucleotide having at least 89% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 5. 
     
     
         5 . The recombinant host cell of  claim 1 , wherein the polypeptide is a fragment of the mature polypeptide of SEQ ID NO: 2, 4 or 6. 
     
     
         6 . The recombinant host cell of  claim 1 , comprising or consisting of SEQ ID NO: 2, 4, 6 or the mature polypeptide of SEQ ID NO: 2, 4 or 6. 
     
     
         7 . The recombinant host cell of  claim 6 , wherein the mature polypeptide is amino acids 1 to 313 of SEQ ID NO: 2, amino acids 1 to 314 of SEQ ID NO: 4 or amino acids 1 to 314 of SEQ ID NO: 6. 
     
     
         8 . A method of producing a polypeptide having protease activity comprising:
 (a) cultivating the recombinant host cell of  claim 1  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         9 . A method of producing a polypeptide having protease activity comprising:
 (a) cultivating the recombinant host cell of  claim 2  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         10 . A method of producing a polypeptide having protease activity comprising:
 (a) cultivating the recombinant host cell of  claim 3  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         11 . A method of producing a polypeptide having protease activity comprising:
 (a) cultivating the recombinant host cell of  claim 4  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         12 . A method of producing a polypeptide having protease activity comprising:
 (a) cultivating the recombinant host cell of  claim 5  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         13 . A method of producing a polypeptide having protease activity comprising:
 (a) cultivating the recombinant host cell of  claim 5  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         14 . A method of producing a polypeptide having protease activity comprising:
 (a) cultivating the recombinant host cell of  claim 6  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         15 . The method of  claim 7 , wherein the recombinant host cell is a  Bacillus  recombinant host cell. 
     
     
         16 . A detergent composition comprising a surfactant and a polypeptide having protease activity, wherein the polypeptide has at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 2; at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 4; or at least 89% sequence identity to the mature polypeptide of SEQ ID NO: 6. 
     
     
         17 . The detergent composition of  claim 16 , which further comprises one of more additional enzymes selected from the group consisting of amylases, catalases, cellulases, cutinases, haloperoxygenases, lipases, mannanases, pectinases, pectin lyases, peroxidases, proteases, xanthanases, xyloglucanases, and mixtures thereof. 
     
     
         18 . The detergent composition of  claim 16 , further comprising one or more components selected from the group consisting of builders, bleaching systems, polymers and hydrotropes. 
     
     
         19 . The detergent composition of  claim 16 , which is in the form of a bar, a homogenous tablet, a tablet having two or more layers, a pouch having one or more compartments, a regular or compact powder, a granule, a paste, a gel, or a regular, compact or concentrated liquid. 
     
     
         20 . A method of cleaning laundry or a hard surface, comprising treating the laundry or hard surface with a polypeptide having protease activity, wherein the polypeptide has at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 2; at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 4; or at least 89% sequence identity to the mature polypeptide of SEQ ID NO: 6.