Methods and compositions for expression cassettes with substituted elements for enhanced expression

Abstract

In one aspect, the present invention provides an expression cassette comprising a promoter operably linked to a nucleic acid molecule of interest, which is operably linked to a terminator, further comprising a heterologous intron that enhances expression of the nucleic acid molecule of interest, wherein the heterologous intron is selected from the group comprised of SEQUENCE ID NO. 1 (iZm10430-02), SEQUENCE ID NO. 2 (iZm10430-01) SEQUENCE ID NO. 3 (iZm008975-01), SEQUENCE ID NO. 4 (iZm005854-01), SEQUENCE ID NO. 5 (iZm010719-01), and SEQUENCE ID NO. 6 (iZm007840-01). In another aspect, the present invention provides an expression cassette comprising a promoter operably linked to a nucleic acid molecule of interest, which is operably linked to a heterologous terminator that enhances expression of the nucleic acid molecule of interest, wherein the heterologous terminator is selected from the group of SEQUENCE ID NO. 7 (tZmHSP70-01), SEQUENCE ID NO. 8 (tZmUbi158-01), SEQUENCE ID NO. 9 (tZmUbi1-01), SEQUENCE ID NO. 10 (tZmUbi361-01).

What is claimed is: 
     
         1 . An expression cassette comprising a promoter operably linked to a nucleic acid molecule of interest, which is operably linked to a terminator, further comprising a heterologous intron that enhances expression of the nucleic acid molecule of interest, wherein the heterologous intron is selected from the group comprised of SEQUENCE ID NO. 1, SEQUENCE ID NO. 2, SEQUENCE ID NO. 3, SEQUENCE ID NO. 4, SEQUENCE ID NO. 5, and SEQUENCE ID NO. 6. 
     
     
         2 . The expression cassette of  claim 1  wherein the promoter and terminator originate from a common gene locus. 
     
     
         3 . A recombinant vector comprising the expression cassette of  claim 1 . 
     
     
         4 . A transgenic plant cell comprising the expression cassette of  claim 1 . 
     
     
         5 . A transgenic plant or progeny thereof comprising the transgenic plant cell of  claim 4 . 
     
     
         6 . A transgenic plant according to  claim 5 , wherein said plant is a monocot plant. 
     
     
         7 . A transgenic plant according to  claim 6 , wherein said plant is a maize plant. 
     
     
         8 . Seed from the transgenic plant of  claim 7 . 
     
     
         9 . An expression cassette comprising a promoter operably linked to a nucleic acid molecule of interest, which is operably linked to a heterologous terminator that enhances expression of the nucleic acid molecule of interest, wherein the heterologous terminator is selected from the group of SEQUENCE ID NO. 7, SEQUENCE ID NO. 8, SEQUENCE ID NO. 9, and SEQUENCE ID NO. 10. 
     
     
         10 . A recombinant vector comprising the expression cassette of  claim 9 . 
     
     
         11 . A monocot transgenic plant cell comprising the expression cassette of  claim 9 . 
     
     
         12 . A method of producing a protein in a plant cell comprising introducing the expression cassette of  claim 1  into the plant cell under conditions whereby the nucleic acid molecule of interest is expressed to produce a protein. 
     
     
         13 . A method of producing a protein in a plant cell comprising introducing the expression cassette of  claim 9  into the plant cell under conditions whereby the nucleic acid molecule of interest is expressed to produce a protein.