Novel Peppers with Unique Aroma and Taste

Abstract

This disclosure concerns the improvement of flavor in peppers by addition of aromas from other or the same pepper species. The disclosure describes novel Capsicum germplasm with aromatic components from Capsicum chinense and other horticultural traits from Capsicum annuum. The disclosure also describes a method for improving the flavor of a pepper through metabolomic-assisted selection to produce a pepper fruit with desirable aromas. The disclosure further provides multiple quantitative trait loci (QTLs), associated markers, and their use for genetic breeding of pepper plants with desired sweetness and flavor.

1 - 20 . (canceled) 
     
     
         21 . A  Capsicum  seed, plant grown therefrom, or part of either, wherein pepper fruit at or near maturity obtained from said seed or plant or part have a pericarp comprising a total sugar content of at least 5.5%, and one or more of alpha-Cubebene at a level having a Relative GC/MS Response Score of at least 0.05, delta-Cadinene at a level having a Relative GC/MS Response Score of at least 0.1, or 1,4-Cadinadiene at a level having a Relative GC/MS Response Score of at least 0.05. 
     
     
         22 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 , wherein said pericarp comprises a titratable acidity at a higher level than that of the pericarp of hybrid pepper PS09954859 grown under similar conditions. 
     
     
         23 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 , wherein said pericarp comprises a titratable acidity of at least 4.2 mmol H + /100 grams fresh tissue. 
     
     
         24 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 , wherein said  Capsicum  seed is a sweet bell pepper seed. 
     
     
         25 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 , wherein said pepper fruit comprises a pungency of below 5 Scoville Heat Units (SHUs). 
     
     
         26 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 , wherein said  Capsicum  seed is a hybrid. 
     
     
         27 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 , wherein at least 70% of the nuclear genetic material of said  Capsicum  seed is from a  C. annuum  background. 
     
     
         28 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 , wherein less than 30% of the nuclear genetic material of said  Capsicum  seed is from a  C. chinense  background. 
     
     
         29 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 , wherein said  Capsicum  seed is  Capsicum annuum  line ZSP8T14-6274, or SVPS2625, wherein a representative sample of seed of said  Capsicum annuum  line ZSP8T14-6274 has been deposited at ATCC under Accession No. PTA-122300, and wherein a representative sample of seed of said  Capsicum annuum  line SVPS2625 has been deposited at ATCC under Accession No. PTA-122296. 
     
     
         30 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 ; wherein said seed, plant, or part is a  Capsicum annuum  seed, plant, or part; wherein said seed or plant or part is heterozygous for a SMO2 QTL; wherein said SMO2 QTL is identifiable by flanking markers SEQ ID NO: 1 and 2 and is obtainable from the  Capsicum  annum line SVPS2625, a representative sample seed of the  Capsicum annuum  line SVPS2625 having been deposited at ATCC under Accession No. PTA-122296. 
     
     
         31 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 ; wherein said seed, plant, or part is a  Capsicum annuum  seed, plant, or part; wherein said seed or plant or part is heterozygous for a SMO3 QTL; wherein said SMO3 QTL is identifiable by flanking markers SEQ ID NO: 3 and 4 and is obtainable from the  Capsicum annuum  line SVPS2625, a representative sample seed of the  Capsicum annuum  line SVPS2625 having been deposited at ATCC under Accession No. PTA-122296. 
     
     
         32 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 , wherein said  Capsicum  seed is an F 1  progeny of a first parent selected from the group consisting of  Capsicum  line ZSP8T14-6274 and  Capsicum  line SVPS2625, wherein a representative sample of seed of said  Capsicum  line ZSP8T14-6274 has been deposited at ATCC under Accession No. PTA-122300, and wherein a representative sample of seed of said  Capsicum  line SVPS2625 has been deposited at ATCC under Accession No. PTA-122296. 
     
     
         33 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 32 , wherein said seed, plant grown therefrom, or part of either is heterozygous for a SMO2 QTL; wherein said SMO2 QTL is identifiable by marker SEQ ID NO: 1 or 2 and is obtainable from the  Capsicum  line SVPS2625, a representative sample seed of the  Capsicum  line SVPS2625 having been deposited under Accession No. PTA-122296. 
     
     
         34 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 32 , wherein said seed, plant grown therefrom, or part of either is heterozygous for a SMO3 QTL; wherein said SMO3 QTL is identifiable by marker SEQ ID NO: 3 or 4 and is obtainable from the  Capsicum  line SVPS2625, a representative sample seed of the  Capsicum  line SVPS2625 having been deposited under Accession No. PTA-122296. 
     
     
         35 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 32 , wherein said part is an ovule or pollen. 
     
     
         36 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 32 , wherein the second parent of said F 1  progeny is:
 a. a plant of an elite variety selected from the group consisting of Aleppo, Anaheim, ancho, bell, cascabel, cayenne, chilaca, chiltepin, cubanelle, de arbol, dandicut, Fresno, guajillo, Hungarian wax, Italian sweet, jalapeño, Japanese, mirasol, macho, mulato, New Mexico, pasilla, pepperoncini, piquin, pimento, poblano, puya, Serrano, and Tientsin; or   b. a plant of a  Capsicum annuum  variety, wherein the pericarp obtained from a fruit of said  Capsicum annuum  variety comprises a total sugar content of at least 5.5%.   
     
     
         37 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 , wherein pepper fruit at or near maturity obtained from said  Capsicum  seed or plant or part have a pericarp which comprises two or more of alpha-Cubebene at a level having a Relative GC/MS Response Score of at least 0.05, delta-Cadinene at a level having a Relative GC/MS Response Score of at least 0.1, or 1,4-Cadinadiene at a level having a Relative GC/MS Response Score of at least 0.05. 
     
     
         38 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 , wherein pepper fruit at or near maturity obtained from said  Capsicum  seed or plant or part have a pericarp which comprises alpha-Cubebene at a level having a Relative GC/MS Response Score of at least 0.05, delta-Cadinene at a level having a Relative GC/MS Response Score of at least 0.1, and 1,4-Cadinadiene at a level having a Relative GC/MS Response Score of at least 0.05. 
     
     
         39 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 , wherein pepper fruit at or near maturity obtained from said  Capsicum  seed or plant or part have a pericarp which comprises one or more of alpha-Cubebene at a level having a Relative GC/MS Response Score of at least 0.25, delta-Cadinene at a level having a Relative GC/MS Response Score of at least 0.25, or 1,4-Cadinadiene at a level having a Relative GC/MS Response Score of at least 0.15. 
     
     
         40 . A  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 , wherein pepper fruit at or near maturity obtained from said  Capsicum  seed or plant or part have a pericarp which comprises two or more of alpha-Cubebene at a level having a Relative GC/MS Response Score of at least 0.25, delta-Cadinene at a level having a Relative GC/MS Response Score of at least 0.25, or 1,4-Cadinadiene at a level having a Relative GC/MS Response Score of at least 0.15. 
     
     
         41 . The  Capsicum  seed, plant grown therefrom, or part of either, according to  claim 21 , wherein pepper fruit at or near maturity obtained from said  Capsicum  seed or plant or part have a pericarp which comprises alpha-Cubebene at a level having a Relative GC/MS Response Score of at least 0.25, delta-Cadinene at a level having a Relative GC/MS Response Score of at least 0.25, and 1,4-Cadinadiene at a level having a Relative GC/MS Response Score of at least 0.15.