Biofilm disrupting composition

Abstract

A biofilm disrupting composition for use in treating biofilm-mediated infections due to non-Pseudomonas micro-organisms in the Cystic Fibrosis patient. One embodiment of the composition of the invention comprises at least one biologically acceptable thiol based antioxidant and at least one antibiotic. Another embodiment of the composition of the invention comprises at least one biologically acceptable thiol based antioxidant, at least one enzyme and at least one antibiotic. The invention is also directed to the process of preparing the composition of the invention, the use of the composition for the manufacture of a medicament for disrupting biofilms formed by non-Pseudomonad micro-organisms, and a method of disrupting biofilm formed by non-Pseudomonad micro-organisms in a patient, comprising administering to the patient the composition of the invention.

1 - 20 . (canceled) 
     
     
         21 . A biofilm disrupting composition when used for disrupting biofilms formed by non-Pseudomonad micro-organisms comprising:
 (a) at least one biologically acceptable thiol based antioxidant, and   (b) at least one antibiotic.   
     
     
         22 . The composition according to  claim 21  wherein the biologically acceptable thiol based antioxidant is selected from the group consisting of mercaptoethanol, N-acetyl cysteine, glutathione, thiamphenicol glycinate, acetylcysteinate, sodium mercaptoethane sulfonate, lipoic acid and erdosteine. 
     
     
         23 . The composition according to  claim 22  wherein the biologically acceptable thiol based antioxidant is glutathione. 
     
     
         24 . The composition according to  claim 21  wherein the antibiotic is selected from the group consisting of antibiotic classes Penicillins, Tetracyclines, Cephalosporins, Quinolones, Lincomycins, Macrolides, Sulfonamides, Glycopeptides, Aminoglycosides and Carbapenems and mixtures thereof. 
     
     
         25 . The composition according to  claim 24  wherein the antibiotic is selected from the group consisting of ciprofloxacin, dexamethasone, amoxicillin/clavulanate, cefixime, cefaclor, clarithromycin, levofloxacin, moxifloxacin and telithromycin. 
     
     
         26 . A biofilm disrupting composition when used for disrupting biofilms formed by non-Pseudomonad micro-organisms comprising:
 (a) at least one biologically acceptable thiol based antioxidant,   (b) at least one enzyme and   (c) at least one antibiotic.   
     
     
         27 . The composition according to  claim 26  wherein the biologically acceptable thiol based antioxidant is selected from the group consisting of mercaptoethanol, N-acetyl cysteine, glutathione, thiamphenicol glycinate, acetylcysteinate, sodium mercaptoethane sulfonate, lipoic acid and erdosteine. 
     
     
         28 . The composition according to  claim 27  wherein the biologically acceptable thiol based antioxidant is glutathione. 
     
     
         29 . The composition according to  claim 26  wherein the antibiotic is selected from the group consisting of antibiotic classes Penicillins, Tetracyclines, Cephalosporins, Quinolones, Lincomycins, Macrolides, Sulfonamides, Glycopeptides, Aminoglycosides and Carbapenems and mixtures thereof. 
     
     
         30 . The composition according to  claim 29  wherein the antibiotic is selected from the group consisting of ciprofloxacin, dexamethasone, amoxicillin/clavulanate, cefixime, cefaclor, clarithromycin, levofloxacin, moxifloxacin and telithromycin. 
     
     
         31 . The composition according to  claim 26  wherein the enzyme is selected from the group consisting of DNase, amylase, cellulase, and proteinase. 
     
     
         32 . The composition according to  claim 31  wherein the enzyme is DNase. 
     
     
         33 . A process of preparing a biofilm disrupting composition according to  claim 21 , which process comprises combining at least one biologically acceptable thiol based antioxidant and at least one antibiotic, to form said composition. 
     
     
         34 . A process of preparing a biofilm disrupting composition according to  claim 26 , which process comprises combining at least one biologically acceptable thiol based antioxidant, at least one antibiotic and at least one enzyme, to form said composition. 
     
     
         35 . The use of a composition comprising:
 (a) at least one biologically acceptable thiol based antioxidant, and   (b) at least one antibiotic,   for the manufacture of a medicament for disrupting biofilms formed by non-Pseudomonad micro-organisms.   
     
     
         36 . The use according to  claim 35  wherein the biologically acceptable thiol based antioxidant is selected from the group consisting of mercaptoethanol, N-acetyl cysteine, glutathione, thiamphenicol glycinate, acetylcysteinate, sodium mercaptoethane sulfonate, lipoic acid and erdosteine. 
     
     
         37 . The use according to  claim 36  wherein the biologically acceptable thiol based antioxidant is glutathione. 
     
     
         38 . The use according to  claim 35  wherein the antibiotic is selected from the group consisting of antibiotic classes Penicillins, Tetracyclines, Cephalosporins, Quinolones, Lincomycins, Macrolides, Sulfonamides, Glycopeptides, Aminoglycosides and Carbapenems and mixtures thereof. 
     
     
         39 . The use according to  claim 38  wherein the antibiotic is selected from the group consisting of ciprofloxacin, dexamethasone, amoxicillin/clavulanate, cefixime, cefaclor, clarithromycin, levofloxacin, moxifloxacin and telithromycin. 
     
     
         40 . The use according to  claim 39  wherein the enzyme is selected from the group consisting of DNase, amylase, cellulase, and proteinase. 
     
     
         41 . The use of a composition comprising:
 (a) at least one biologically acceptable thiol based antioxidant,   (b) at least one enzyme and   (c) at least one antibiotic,   for the manufacture of a medicament for disrupting biofilms formed by non-Pseudomonad micro-organisms.   
     
     
         42 . The use according to  claim 41  wherein the biologically acceptable thiol based antioxidant is selected from the group consisting of mercaptoethanol, N-acetyl cysteine, glutathione, thiamphenicol glycinate, acetylcysteinate, sodium mercaptoethane sulfonate, lipoic acid and erdosteine. 
     
     
         43 . The use according to  claim 42  wherein the biologically acceptable thiol based antioxidant is glutathione. 
     
     
         44 . The use according to  claim 41  wherein the antibiotic is selected from the group consisting of antibiotic classes Penicillins, Tetracyclines, Cephalosporins, Quinolones, Lincomycins, Macrolides, Sulfonamides, Glycopeptides, Aminoglycosides and Carbapenems and mixtures thereof. 
     
     
         45 . The use according to  claim 44  wherein the antibiotic is selected from the group consisting of ciprofloxacin, dexamethasone, amoxicillin/clavulanate, cefixime, cefaclor, clarithromycin, levofloxacin, moxifloxacin and telithromycin. 
     
     
         46 . The use according to  claim 45  wherein the enzyme is selected from the group consisting of DNase, amylase, cellulase, and proteinase. 
     
     
         47 . A method of disrupting biofilm formed by non-Pseudomonad micro-organisms in a patient, which method comprises administering to said patient a composition according to  claim 21  in an amount which effectively disrupts said biofilm. 
     
     
         48 . A method of disrupting biofilm formed by non-Pseudomonad micro-organisms in a patient, which method comprises administering to said patient a composition according to  claim 26  in an amount which effectively disrupts said biofilm.