US2020023001A1PendingUtilityA1

GlycoFix (Structurally And Functionally Repaired Endothelial Glycocalyx)

Assignee: UNIV NORTHEASTERNPriority: Jul 20, 2018Filed: Jul 19, 2019Published: Jan 23, 2020
Est. expiryJul 20, 2038(~11.9 yrs left)· nominal 20-yr term from priority
A61L 2300/45A61L 2300/236A61L 2300/22A61L 31/16A61L 29/16A61L 27/54A61L 27/507A61P 9/10A61K 31/661A61K 31/737A61K 9/0024
48
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Claims

Abstract

Provided herein are compositions comprising heparan sulfate, or a pharmaceutically acceptable salt thereof, and sphingosine-1-phosphate, or a pharmaceutically acceptable salt thereof. Such compositions are useful in a variety of methods, including methods of regenerating endothelial glycocalyx, preventing endothelial glycocalyx degradation and treating vascular disease.

Claims

exact text as granted — not AI-modified
1 . A composition, comprising heparan sulfate, or a pharmaceutically acceptable salt thereof, and sphingosine-1-phosphate, or a pharmaceutically acceptable salt thereof. 
     
     
         2 . The composition of  claim 1 , wherein the heparan sulfate is exogenous heparan sulfate. 
     
     
         3 . The composition of  claim 2 , wherein the heparan sulfate is porcine mucosal heparan sulfate with an average molecular weight of about 15 kDa. 
     
     
         4 . A method of regenerating glycocalyx of a cell, comprising contacting the cell with an effective amount of heparan sulfate, or a pharmaceutically acceptable salt thereof, and an effective amount of sphingosine-1-phosphate, or a pharmaceutically acceptable salt thereof. 
     
     
         5 - 9 . (canceled) 
     
     
         10 . The method of  claim 4 , wherein the cell is an endothelial cell. 
     
     
         11 . A method of treating a vascular disease in a subject in need thereof, comprising administering to the subject a therapeutically effective amount of heparan sulfate, or a pharmaceutically acceptable salt thereof, and a therapeutically effective amount of sphingosine-1-phosphate, or a pharmaceutically acceptable salt thereof. 
     
     
         12 . The method of  claim 11 , wherein the vascular disease is atherosclerosis, a blood clot, a stroke, peripheral artery disease, an aneurysm, a pulmonary embolism, carotid artery disease, arteriovenous malformation, critical limb ischemia, deep vein thrombosis, chronic venous insufficiency, a varicose vein, coronary artery disease, Raynaud's disease or vasculitis. 
     
     
         13 . The method of  claim 12 , wherein the vascular disease is atherosclerosis. 
     
     
         14 . The method of  claim 4 , wherein the heparan sulfate is exogenous heparan sulfate. 
     
     
         15 . The method of  claim 14 , wherein the heparan sulfate is porcine mucosal heparan sulfate with an average molecular weight of about 15 kDa. 
     
     
         16 . An implant, comprising the composition of  claim 1 . 
     
     
         17 . The implant of  claim 16 , wherein the implant is a stent or catheter. 
     
     
         18 . The implant of  claim 16 , wherein the heparan sulfate, or a pharmaceutically acceptable salt thereof, and the sphingosine-1-phosphate, or a pharmaceutically acceptable salt thereof, are coated on a surface of the implant. 
     
     
         19 . The implant of  claim 16 , wherein the heparan sulfate is porcine mucosal heparan sulfate with an average molecular weight of about 15 kDa. 
     
     
         20 . A vascular graft, comprising the composition of  claim 1 . 
     
     
         21 . The vascular graft of  claim 20 , wherein the heparan sulfate is porcine mucosal heparan sulfate with an average molecular weight of about 15 kDa. 
     
     
         22 - 24 . (canceled)

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