Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

Abstract

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.

1 - 19 . (canceled) 
     
     
         20 . A recombinant host cell comprising a nucleic acid construct comprising a polynucleotide encoding a GH61 polypeptide having cellulolytic enhancing activity operably linked to one or more control sequences that direct the production of the GH61 polypeptide, wherein the GH61 polypeptide having cellulolytic enhancing activity is heterologous to the recombinant host cell, and wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from the group consisting of:
 (a) a GH61 polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of amino acids 20 to 336 of SEQ ID NO: 10;   (b) a GH61 polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence of nucleotides 58 to 1008 of SEQ ID NO: 9;   (c) a GH61 polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of amino acids 20 to 251 of SEQ ID NO: 10; and   (d) a GH61 polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence of nucleotides 58 to 753 of SEQ ID NO: 9.   
     
     
         21 . The recombinant host cell of  claim 20 , wherein the GH61 polypeptide having cellulolytic enhancing activity comprises an amino acid sequence having at least 96% sequence identity to the amino acid sequence of amino acids 20 to 336 of SEQ ID NO: 10 or amino acids 20 to 251 of SEQ ID NO: 10. 
     
     
         22 . The recombinant host cell of  claim 20 , wherein the GH61 polypeptide having cellulolytic enhancing activity comprises an amino acid sequence having at least 97% sequence identity to the amino acid sequence of amino acids 20 to 336 of SEQ ID NO: 10 or amino acids 20 to 251 of SEQ ID NO: 10. 
     
     
         23 . The recombinant host cell of  claim 20 , wherein the GH61 polypeptide having cellulolytic enhancing activity comprises an amino acid sequence having at least 98% sequence identity to the amino acid sequence of amino acids 20 to 336 of SEQ ID NO: 10 or amino acids 20 to 251 of SEQ ID NO: 10. 
     
     
         24 . The recombinant host cell of  claim 20 , wherein the GH61 polypeptide having cellulolytic enhancing activity comprises an amino acid sequence having at least 99% sequence identity to the amino acid sequence of amino acids 20 to 336 of SEQ ID NO: 10 or amino acids 20 to 251 of SEQ ID NO: 10. 
     
     
         25 . The recombinant host cell of  claim 20 , wherein the GH61 polypeptide having cellulolytic enhancing activity comprises the amino acid sequence of SEQ ID NO: 10, the amino acid sequence of amino acids 20 to 336 of SEQ ID NO: 10, or the amino acid sequence of amino acids 20 to 251 of SEQ ID NO: 10. 
     
     
         26 . A method of producing a GH61 polypeptide having cellulolytic enhancing activity, comprising:
 (a) cultivating the recombinant host cell of  claim 20  under conditions conducive for production of the polypeptide; and optionally   (b) recovering the GH61 polypeptide.   
     
     
         27 . A method of producing a GH61 polypeptide having cellulolytic enhancing activity, comprising:
 (a) cultivating the recombinant host cell of  claim 25  under conditions conducive for production of the polypeptide; and optionally   (b) recovering the GH61 polypeptide.