Detection methods employing hcv core lipid and dna binding domain monoclonal antibodies

Abstract

The present disclosure provides detection methods employing HCV core lipid binding domain and DNA binding domain monoclonal antibodies or antibody fragments. In certain embodiments, the lipid binding domain monoclonal antibody or antibody fragment recognizes an epitope in amino acids 141 to 161 of HCV core protein and the DNA binding domain antibody or antibody fragment recognizes an epitope in amino acids 95-123 (e.g., in amino acids 99-117) of HCV core protein.

1 .- 16 . (canceled) 
     
     
         17 . A system comprising:
 (a) a first antibody, or antigen-binding portion thereof, which comprises heavy chain CDR1, CDR2, and CDR3 amino acid sequences of SEQ ID NO: 588, SEQ ID NO: 589, and SEQ ID NO: 590, respectively, and light chain CDR1, CDR2, and CDR3 amino acid sequences of SEQ ID NO: 585, SEQ ID NO: 586, and SEQ ID NO: 587; and   (b) a second antibody, or antigen-binding portion thereof, which specifically binds to the amino acid sequence of SEQ ID NO: 574 in the lipid binding domain of HCV core protein.   
     
     
         18 .- 21 . (canceled) 
     
     
         22 . The system of  claim 17 , wherein the first and/or second antibody, or the antigen-binding portion thereof, comprises a detectable label attached thereto. 
     
     
         23 . A kit comprising:
 (a) a first antibody, or antigen-binding portion thereof, which comprises heavy chain CDR1, CDR2, and CDR3 amino acid sequences of SEQ ID NO: 588, SEQ ID NO: 589, and SEQ ID NO: 590, respectively, and light chain CDR1, CDR2, and CDR3 amino acid sequences of SEQ ID NO: 585, SEQ ID NO: 586, and SEQ ID NO: 587;   (b) a second antibody, or antigen-binding portion thereof, which specifically binds to the amino acid sequence of SEQ ID NO: 574 in the lipid binding domain of HCV core protein; and   (c) instructions for detecting an HCV core protein in a sample.   
     
     
         24 . The kit of  claim 23 , wherein the first and/or second antibody, or the antigen-binding portion thereof, comprises a detectable label attached thereto.