Cell treatment device, suspension culture vessel, and stem cell induction method

Abstract

This cell treatment device is provided with: a factor introduction device 30 for introducing a pluripotent induction factor into cells so as to prepare induction factor-introduced cells; and a reprogramming suspension culture vessel for culturing the induction factor-introduced cells that have been prepared by the factor introduction device 30.

1 . A cell treatment device comprising:
 a factor introducing device that introduces a pluripotency inducing factor into cells to create inducing factor-introduced cells, and   a suspension culture vessel for suspension culturing of the inducing factor-introduced cells created by the factor introducing device, to induce induced pluripotent stem cells from the inducing factor-introduced cells.   
     
     
         2 . The cell treatment device according to  claim 1 , wherein the inducing factor-introduced cells are reprogrammed inside the suspension culture vessel. 
     
     
         3 . The cell treatment device according to  claim 1 , wherein the induced cells are amplifying cultured inside the suspension culture vessel. 
     
     
         4 . The cell treatment device according to  claim 1 , wherein the suspension culture vessel comprises:
 a semipermeable membrane in which the inducing factor-introduced cells and culture medium are placed,   a container in which the semipermeable membrane is placed with the culture medium accommodated around the periphery of the semipermeable membrane, and   a drive unit for agitation of the culture medium in the container.   
     
     
         5 - 6 . (canceled) 
     
     
         7 . The cell treatment device according to  claim 1 , wherein the suspension culture vessel comprises:
 a semipermeable membrane in which the inducing factor-introduced cells and culture medium are placed,   a container in which the semipermeable membrane is placed with the culture medium accommodated around the periphery of the semipermeable membrane, and with a helical groove provided on its inner surface, and   a pump that supplies culture medium into the container so that the culture medium flows along the groove inside the container.   
     
     
         8 . The cell treatment device according to  claim 4 , wherein the inducing factor-introduced cells created by the factor introducing device are fed into the semipermeable membrane of the suspension culture vessel. 
     
     
         9 . The cell treatment device according to  claim 1 , wherein the suspension culture vessel comprises:
 a semipermeable membrane in which the culture medium is placed,   a container in which the semipermeable membrane is placed with the inducing factor-introduced cells and culture medium accommodated around the periphery of the semipermeable membrane, and   a pump that supplies culture medium into the semipermeable membrane so as to create a flow of the culture medium inside the semipermeable membrane.   
     
     
         10 . The cell treatment device according to  claim 9 , wherein the inducing factor-introduced cells created by the factor introducing device are fed outside of the semipermeable membrane inside the container. 
     
     
         11 . The cell treatment device according to  claim 1 , wherein the suspension culture vessel comprises:
 a container in which the inducing factor-introduced cells and culture medium are placed, and   a drive unit for agitation of the culture medium in the container.   
     
     
         12 - 13 . (canceled) 
     
     
         14 . The cell treatment device according to  claim 1 , wherein the suspension culture vessel comprises:
 a container in which the inducing factor-introduced cells and culture medium are placed, the container being provided with a helical groove provided on its inner surface, and   a pump that supplies culture medium into the container so that the medium flows along the groove inside the container.   
     
     
         15 - 17 . (canceled) 
     
     
         18 . A suspension culture vessel comprising:
 a semipermeable membrane in which inducing factor-introduced cells, in which an inducing factor has been introduced, and culture medium are placed,   a solution-feeding channel for feeding of culture medium containing the inducing factor-introduced cells into the semipermeable membrane,   a container in which the semipermeable membrane is placed, with the culture medium accommodated around the periphery of the semipermeable membrane, and   an agitating member provided inside the container,   and being for suspension culturing of the inducing factor-introduced cells in the semipermeable membrane to induce induced pluripotent stem cells from the inducing factor-introduced cells.   
     
     
         19 . The suspension culture vessel according to  claim 18 , which further comprises a drive unit for agitation of the culture medium in the container. 
     
     
         20 . (canceled) 
     
     
         21 . A suspension culture vessel comprising:
 a semipermeable membrane in which inducing factor-introduced cells, in which an inducing factor has been introduced, and culture medium are placed,   a solution-feeding channel for feeding of culture medium containing the inducing factor-introduced cells into the semipermeable membrane, and   a container in which the semipermeable membrane is placed, with the culture medium accommodated around the periphery of the semipermeable membrane and with a helical groove provided on its inner surface,   and being for suspension culturing of the inducing factor-introduced cells in the semipermeable membrane to induce induced pluripotent stem cells from the inducing factor-introduced cells.   
     
     
         22 - 24 . (canceled) 
     
     
         25 . A suspension culture vessel comprising:
 a semipermeable membrane in which culture medium is placed, and   a container in which the semipermeable membrane is placed, with inducing factor-introduced cells in which an inducing factor has been introduced, and culture medium, accommodated around the periphery of the semipermeable membrane,   and being for suspension culturing of the inducing factor-introduced cells in the container to induce induced pluripotent stem cells from the inducing factor-introduced cells.   
     
     
         26 . The suspension culture vessel according to  claim 25 , which further comprises a pump that supplies culture medium into the semipermeable membrane so as to create a flow of the culture medium inside the semipermeable membrane. 
     
     
         27 - 28 . (canceled) 
     
     
         29 . A stem cell induction method that includes:
 introducing a pluripotency inducing factor into cells to create inducing factor-introduced cells, and   suspension culturing the inducing factor-introduced cells to induce induced pluripotent stem cells from the inducing factor-introduced cells.   
     
     
         30 . The stem cell induction method according to  claim 29 , wherein the suspension culture is agitation culture. 
     
     
         31 - 36 . (canceled) 
     
     
         37 . The cell treatment device according to  claim 1 , wherein the suspension culture vessel comprises a semipermeable membrane. 
     
     
         38 . The cell treatment device according to  claim 37 , wherein the inducing factor-introduced cells created by the factor introducing device are fed to the suspension culture vessel.