Cell cryopreservation composition and cryopreservation method

Abstract

This composition can be easily added when cryopreserving cells and improves cell viability. 1 volume % of the composition containing 0.01 wt % to 20 wt % of a sophorose lipid is added just before or up to 6 hours before cryopreservation and cells are stored. This composition can improve cryopreservation cell viability as it reduces freezing damage to cells. With this composition, cells can be stored without using DMSO and blood serum. This is a simple and inexpensive storage method as it does not require fine control of the freezing rate during cell cryopreservation.

1 . A composition comprising 0.01 wt % to 20 wt % of a sophorose lipid, wherein, when added to cells, the composition improves cell viability after cryopreservation. 
     
     
         2 . The composition of  claim 1 , comprising 5 wt % to 10 wt % of dimethyl sulfoxide (DMSO). 
     
     
         3 . The composition of  claim 1 , comprising 1 wt % to 50 wt % of a polyhydric alcohol. 
     
     
         4 . The composition of  claim 3 , comprising at least one of glycerin, ethylene glycol, and propylene glycol as the polyhydric alcohol. 
     
     
         5 . A composition comprising a sophorose lipid and a polyhydric alcohol, wherein the composition does not contain dimethyl sulfoxide (DMSO), and wherein the composition, when added to cells, improves cell viability after cryopreservation. 
     
     
         6 . The composition of  claim 5 , wherein the polyhydric alcohol is glycerin, and the cells are stem cells. 
     
     
         7 . The composition of  claim 6 , wherein the stem cells are mesenchymal stem cells. 
     
     
         8 . A cell cryopreservation method comprising adding a 1 volume % of the composition of  claim 1  to cells in a cell culture medium just before or up to 6 hours before cryopreserving the cells. 
     
     
         9 . A cell cryopreservation method comprising adding a 10 volume % to 99 volume % of the composition of  claim 2  to cells in a cell culture medium when cryopreserving cells.