Active substance of lactobacillus plantarum gkm3, a composition comprising thereof and its use for promoting longevity

Abstract

The present invention provides an active substance of Lactic Acid Bacteria, a composition comprising thereof and its use for promoting longevity, especially for increasing Cisd2 gene expression, reducing mitochondrial damage and delaying aging conditions such as nerve degeneration and sarcopenia.

1 . A longevity-promoting composition comprising a  Lactobacillus plantarum , wherein the  Lactobacillus plantarum  is deposited (No. 14565) at China General Microbiological Culture Collection Center. 
     
     
         2 . A longevity-promoting composition comprising an active substance of  Lactobacillus plantarum  of an effective amount, wherein the  Lactobacillus plantarum  is deposited (No. 14565) at China General Microbiological Culture Collection Center. 
     
     
         3 . The composition of  claim 2 , wherein the active substance is prepared by the following method:
 (a) streaking lactic acid bacteria on a solid-state media to produce single colonies; and   (b) inoculating a single colony of lactic acid bacteria cultured at step (a) to a liquid culture for liquid-state culture.   
     
     
         4 . The composition of  claim 3 , wherein said method further comprises the following steps:
 (c) centrifuging the liquid culture cultured at step (b) to obtain a bacterial pellet; and   (d) freeze-drying the bacterial pellet obtained at step (c).   
     
     
         5 . The composition of  claim 3 , wherein step (b) is carried out at 30 to 50° C., in nitrogen or carbon dioxide of 0 to 1 VVM, rotate at 10 to 100 rpm, and/or incubate for 16 to 24 hours. 
     
     
         6 . The composition of  claim 4 , wherein the temperature for the freeze-drying at step (d) is −196 to −40° C. 
     
     
         7 . The composition of  claim 1 , the composition comprising an additive selected from the group consisting of an excipient, preservative, diluent, filler, absorption enhancer, sweetener and a combination thereof. 
     
     
         8 . The composition of  claim 2 , the composition comprising an additive selected from the group consisting of: an excipient, preservative, diluent, filler, absorption enhancer, sweetener and a combination thereof. 
     
     
         9 . The composition of  claim 1 , the composition being a drug, feed, drink, nutritional supplement, dairy product or health food. 
     
     
         10 . The composition of  claim 2 , the composition being a drug, feed, drink, nutritional supplement, dairy product or health food. 
     
     
         11 . The composition of  claim 1 , the composition taking the form of a powder, tablet, granule, suppository, microcapsule, ampoule/ampule, liquid spray or suppository form. 
     
     
         12 . The composition of  claim 2 , the composition taking the form of a powder, tablet, granule, suppository, microcapsule, ampoule/ampule, liquid spray or suppository form. 
     
     
         13 . A method for manufacturing a composition of  claim 1  for promoting longevity. 
     
     
         14 . The method of  claim 13 , wherein for promoting longevity, a subject having been administered the active substance exhibits increased Cisd2 gene expression compared to a subject that has not been administered the active substance. 
     
     
         15 . The method of  claim 13 , wherein for promoting longevity, a subject having been administered the active substance exhibits reduced and delayed mitochondrial damage compared to a subject that has not been administered the active substance. 
     
     
         16 . The method of  claim 13 , wherein for promoting longevity, a subject having been administered the active substance exhibits delayed aging conditions of nerve degeneration, sarcopenia or a combination thereof, compared to a subject that has not been administered the active substance. 
     
     
         17 . A method for manufacturing a composition of  claim 2  for promoting longevity.