Pneumococcal fusion protein vaccines

Abstract

Technologies for the prevention and/or treatment of pneumococcal infections.

1 . A fusion protein comprising:
 (i) a biotin-binding moiety;   (ii) an SP1500 polypeptide or fragment; and   (iii) an SP0785 polypeptide or fragment.   
     
     
         2 . A fusion protein comprising:
 (i) a biotin-binding moiety comprising an amino acid sequence at least 80%, 85%, 90% identical to SEQ ID NO:2 or a biotin binding portion thereof;   (ii) a polypeptide comprising an amino acid sequence at least 80%, 85%, 90% identical to SEQ ID NO:7 or an antigenic portion thereof; and   (iii) a polypeptide comprising an amino acid sequence at least 80%, 85%, 90% identical to SEQ ID NO:4 or an antigenic portion thereof.   
     
     
         3 . A fusion protein comprising:
 (i) a biotin-binding moiety comprising an amino acid sequence at least 80%, 85%, 90% identical to SEQ ID NO:2 or a biotin binding portion thereof;   (ii) a first polypeptide comprising an amino acid sequence at least 80%, 85%, 90% identical to SEQ ID NO:7 or an antigenic portion thereof;   (iii) a second polypeptide comprising an amino acid sequence at least 80%, 85%, 90% identical to SEQ ID NO:4 or an antigenic portion thereof, and   (iv) a first linker positioned between the biotin-binding moiety and the first polypeptide and/or a second linker positioned between the first polypeptide and the second polypeptide.   
     
     
         4 . The fusion protein of  claim 3 , wherein the first linker comprises the amino acid sequence GGGGSSS (SEQ ID NO: 37). 
     
     
         5 . The fusion protein of  claim 3  or  claim 4 , wherein the second linker comprises the amino acid sequence AAA (SEQ ID NO: 38). 
     
     
         6 . The fusion protein of any one of  claims 1 - 5 , wherein the biotin-binding moiety is C-terminally linked to the first polypeptide. 
     
     
         7 . The fusion protein of any one of  claims 1 - 5 , wherein the biotin-binding moiety is N-terminally linked to the first polypeptide. 
     
     
         8 . The fusion protein of any one of  claims 1 - 7 , wherein the first polypeptide is C-terminally linked to the second polypeptide. 
     
     
         9 . The fusion protein of any one of  claims 1 - 7 , wherein the first polypeptide is N-terminally linked to the second polypeptide. 
     
     
         10 . The fusion protein of any one of  claims 1 - 9 , wherein the second polypeptide is C-terminally linked to the biotin-binding moiety. 
     
     
         11 . The fusion protein of any one of  claims 1 - 9 , wherein the second polypeptide is N-terminally linked to the biotin-binding moiety. 
     
     
         12 . The fusion protein of any one of  claims 1 - 11 , comprising the amino acid sequence of SEQ ID NO:2. 
     
     
         13 . The fusion protein of any one of  claims 1 - 12 , comprising the amino acid sequence of SEQ ID NO:7. 
     
     
         14 . The fusion protein of any one of  claims 1 - 13 , comprising the amino acid sequence of SEQ ID NO:4. 
     
     
         15 . The fusion protein of any one of  claims 1 - 14 , comprising the amino acid sequence at least 80%, 85%, 90% identical to SEQ ID NO:23. 
     
     
         16 . The fusion protein of any one of  claims 1 - 15 , wherein the fusion protein is CP1.