Phytase formulation

Abstract

A liquid enzyme formulation, an enzyme granule formulation, methods for manufacturing enzyme granules using a fluid bed dryer, wherein the enzyme granules are thermostable without the need for a thermostable coating is provided. The enzyme granules are phytase granules used in the manufacturing of an animal feed, wherein the phytase granule is thermostable without the need for a thermostable coating and the phytase retains about 63% to about 134% of its activity after pelleting at 80° C.

What is claimed is: 
     
         1 . A granulated enzyme formulation comprising:
 (a) a carrier;   (b) a primary solution comprising a phytase, a buffer, a stabilizer, a binding agent, a first plasticizer, an anti-microbial, or any combination thereof; and   (c) a secondary solution comprising an enhancer, a second plasticizer, or any combination thereof.   
     
     
         2 . The granulated enzyme formulation of  claim 1 , wherein the carrier is selected from the group consisting of:
 (a) a flour;   (b) a wheat flour;   (c) a bleached flour   (d) a wheat flour and a maltodextrin;   (e) a wheat flour and a pre-gelatinized starch; and   (f) a starch.   
     
     
         3 . The granulated enzyme formulation of  claim 1 , wherein the buffer is a sodium citrate. 
     
     
         4 . The granulated enzyme formulation of  claim 1 , wherein the stabilizer is selected from the group consisting of: a sucrose, a sorbitol, a mannitol, a glycerol, a trehalose, a sodium chloride, a sodium sulphtate, kaolin, aluminum silicate, magnesium silicate, and any combination thereof. 
     
     
         5 . The granulated enzyme formulation of  claim 1 , wherein the binding agent is selected from the group consisting of: a guar gum, a xanthan, a sodium algenate, a locust bean gum, a carrageenan gum, a pre-gelatinized modified starch, a maltodextrin, gelatin, a methyl celluloase, a hydroxypropyl cellulose, a hydroxypropyl methyl celluloase, a carboxymethyl celluloase, and any combination thereof. 
     
     
         6 . The granulated enzyme formulation of  claim 1 , wherein the first plasticizer is selected from the group consisting of: a glycerol, polyethylene glycol, triethyl citrate, triacetin, and acetyl tri ethyl citrate. 
     
     
         7 . The granulated enzyme formulation of  claim 1 , wherein the anti-micorbial is selected from the group consisting of: a potassium sorbate, sodium sorbate, sodium benzoate, sodium propionate, calcium propionate, ammonium propionate, methyl paraben, benzoic acid, sorbic acid, and any combination thereof. 
     
     
         8 . The granulated enzyme formulation of  claim 1 , wherein the enhancer is selected from the group consisting of: pre-gelatinized modified starch, maltodextrin, sodium algenate, carregeenan with CaCl2, carregeenan without CaCl2, guar gum with sodium borate, guar gum without sodium borate, gelatin; methyl cellulase; hydroxypropyl cellulase; hydroxypropyl methyl cellulase; carboxymethyl cellulase; sodium chloride; sodium sulphate; kaolin; aluminum silicate, magnesium silicate, and any combination thereof. 
     
     
         9 . The granulated enzyme formulation of  claim 1 , wherein the second plasticizer is selected from the group consisting of a glycerol, polyethylene glycol, triethyl citrate, triacetin, and acetyl tri ethyl citrate. 
     
     
         10 . The granulated enzyme formulation of  claim 1 , further comprising a flowing aid, wherein the flowing aid is selected from the group consisting of: silicon dioxide, magnesium stearate, kaolin, talc, diatomaceous earth, and any combination thereof. 
     
     
         11 . A method for manufacturing an enzyme granule in a fluidized bed, wherein the enzyme retains at least 63% activity at 75° C. comprising:
 (a) loading a dry carrier in a fluid bed granulator; 
 (b) adding a primary enzyme solution to the dry carrier; and 
 (c) drying the combination of (a) and (b) to a desired moisture content. 
 
     
     
         12 . The method of  claim 11 , further comprising: (i) adding a secondary granulation solution to (a) and (b). 
     
     
         13 . The method of  claim 11 , further comprising adding a flowing aid to the fluid bed dryer or dry blender after drying. 
     
     
         14 . The method of  claim 12 , wherein the secondary granulation solution further comprises a flowing aid. 
     
     
         15 . The method of  claim 11 , wherein the enzyme is selected from the group consisting of: a Phytase, a cellulase, lactase, lipase, protease, catalase, xylanase, beta-glucanase, mannanase, amylase, amidase, epoxide hydrolase, esterase, phospholipase, transaminase, amine oxidase, cellobiohydrolase, ammonia lyase, and any combination thereof. 
     
     
         16 . The method of  claim 11 , wherein the desired moisture content is between about 3% to about 10%. 
     
     
         17 . A formulation for phytase wherein the phytase retains an activity at a temperature comprising:
 (a) at least 63% activity at 80° C.;   (b) at least 58% activity at 85° C.;   (c) at least 43% activity at 88° C.;   (d) at least 27% activity at 90° C.; and   (e) at least 6% activity at 93° C.