Image analysis and measurement of biological samples

Abstract

Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples.

1 - 4 . (canceled) 
     
     
         5 . A method of identifying a cell in a sample containing a plurality of cells, comprising:
 (a) assaying a cell of the plurality of cells for at least one of: (i) the presence of a cell surface antigen; (ii) the amount of a cell surface antigen; or (iii) cell size;   (b) assaying the cell of (a) for at least one of: (i) nuclear size; or (ii) nuclear shape; and   (c) assaying the cell of (a) and (b) for quantitative cell light scatter,   wherein the combination of information from steps (a), (b), and (c) is used to identify the cell in the sample containing a plurality of cells.   
     
     
         6 . The method of  claim 5  wherein the assaying of the cell comprises using a a fluorescence microscope while the cell is in a non-flowing state. 
     
     
         7 . The method of  claim 5  comprising mixing of the sample in a sample chamber of a cuvette. 
     
     
         8 . The method of  claim 5  wherein the assaying of the cell comprises using reference particles. 
     
     
         9 . The method of  claim 8  wherein the references particles comprise microspheres. 
     
     
         10 . The method of  claim 8  wherein the references particles comprise polystyrene. 
     
     
         11 . The method of  claim 7  wherein said mixing of the sample occurs automatically in a sample processing device. 
     
     
         12 . The method of  claim 8  further comprising using a light beam at a wavelength capable of exciting a fluorophore within the reference particles. 
     
     
         13 . The method of  claim 12  wherein the light beam is at the wavelength capable of exciting the fluorophore attached to a second type of reference particles.