Variable Density Tissue Graft Composition and Methods of Making and Using the Same

Abstract

Disclosed are tissue graft compositions made of materials having different densities, methods of making, and methods of treatment for restoring t issues in a patient.

1 - 40 . (canceled) 
     
     
         41 . A method for restoring tissue damage comprising:
 providing a composition comprising a first layer and a second layer, the first layer comprising an extracellular matrix material and a density that differs from the second layer, each of said first layer and said second layer having a first planar surface and a second planar surface, and each of said first and second layers at least partially overlapping the other layer; and,   applying said composition to the damaged tissue.   
     
     
         42 . The method of  claim 41 , wherein said first layer of said composition comprises aponeurosis. 
     
     
         43 . The method of  claim 41 , wherein said first layer comprises a plurality of perforations, each of said perforations comprising a lumen and said second layer is non-perforated, said lumen of the perforations of said first layer enclosing a portion of said second layer whereby said perforated first layer is joined to said non-perforated second layer. 
     
     
         44 . The method of  claim 41 , wherein said first layer comprises epithelial basement membrane. 
     
     
         45 . The method of  claim 44 , wherein said composition is implanted in an abdominal wall of a patient and said epithelial basement membrane is positioned on a peritoneal surface of the abdominal wall. 
     
     
         46 . The method of  claim 41 , wherein said first layer and said second layer has a density that differs from each other in a range of about 400%-125%. 
     
     
         47 . The method of  claim 41 , wherein said first layer and said second layer has a density that differs from each other in a range of about 300%-150%. 
     
     
         48 . The method of  claim 41 , wherein said first layer and said second layer has a density that differs from each other in a range of about 200%-175%. 
     
     
         49 . The method of  claim 41 , wherein said damaged tissue is a joint. 
     
     
         50 . The method of  claim 41 , wherein said damaged tissue is skin. 
     
     
         51 . The method of  claim 41 , wherein said damaged tissue is an abdominal body wall. 
     
     
         52 . The method of  claim 41 , wherein said damaged tissue is a thoracic body wall. 
     
     
         53 . The method of  claim 41 , wherein said damaged tissue is a heart wall. 
     
     
         54 . The method of  claim 41 , wherein said damaged tissue is a vascular vessel wall. 
     
     
         55 . The method of  claim 41 , wherein said damaged tissue is a ligament. 
     
     
         56 . The method of  claim 41 , wherein said damaged tissue is a hernia. 
     
     
         57 . The method of  claim 41 , wherein said damaged tissue is a joint. 
     
     
         58 . The method of  claim 41 , wherein said damaged tissue is a urinary bladder. 
     
     
         59 . The method of  claim 44 , wherein said composition is implanted in a joint of a patient and said epithelial basement membrane is positioned on a synovial surface of said joint. 
     
     
         60 . The method of  claim 44 , wherein said composition is implanted in the wall of a urinary bladder of a patient and said epithelial basement membrane is positioned on the urothelial side of said bladder wall. 
     
     
         61 . The method of  claim 44 , wherein said composition is implanted in the wall of a vessel of a patient and said epithelial basement membrane is positioned on the luminal side of said vessel wall. 
     
     
         62 . The method of  claim 44 , wherein said composition is implanted in the wall of a heart of a patient and said epithelial basement membrane is positioned on the luminal side of said heart wall. 
     
     
         63 . The method of  claim 41 , wherein at least one of said layers of the composition is bioreabsorbed within about 30 days. 
     
     
         64 . The method of  claim 41 , wherein at least one of layers is bioreabsorbed within about 30-120 days. 
     
     
         65 . The method of  claim 41 , wherein said composition further comprises a third layer wherein said first layer is positioned between and joined to said third layer and second layer.