Devices, systems and methods for evaluation of hemostasis

Abstract

Provided are devices systems and methods for evaluation of hemostasis. In some embodiments, an apparatus is disclosed comprising a housing, a plurality of test chambers located in the housing, the plurality of test chambers including chambers configured for measurements via a system that interrogates one or more viscoelastic properties of test samples in the test chambers, wherein the one or more viscoelastic properties is used to characterize dynamics of coagulation and/or fibrinolysis.

1 .- 73 . (canceled) 
     
     
         74 . A method of using a disposable cartridge with a blood testing console, the method comprising:
 receiving in a fixed volume of a disposable cartridge a blood sample to be tested, wherein the disposable cartridge includes a plurality of testing paths arranged in a parallel, each testing path comprising a channel and a test chamber, including a first channel, a second channel, a first test chamber, and a second test chamber;   allowing a first portion of the blood sample to mix with a first reagent or combination of reagents to produce a first test sample;   allowing a second portion of the blood sample to mix with a second reagent or combination of reagents to form a second test sample;   initiating displacement within the first test sample;   interrogating the first test sample in the first test chamber to measure a first change in mechanical properties of the first test sample;   determining, by a processor, a first curve associated with stiffness of the first test sample as a function of time;   determining at least one parameter including a first parameter selected from the group consisting of clot time, clot stiffness, clot formation rate, and lysis time from the first curve;   initiating displacement within the second test sample;   interrogating the second test sample in the second test chamber to measure a second change in mechanical properties of the second test sample;   determining, by the processor, a second curve associated with stiffness of the first test sample as a function of time; and   determining at least one parameter including a second parameter selected from the group consisting of clot time, clot stiffness, clot formation rate, and lysis time from the second curve;   wherein said first parameter, said second parameter, or a combination thereof provides an indication of a state of hemostasis in the subject; and   wherein the interrogation to determine the first parameter, the second parameter, or a combination thereof is based on a viscoelastic measurement of the blood sample.