US2020149105A1PendingUtilityA1
Device preparation using condensed nucleic acid particles
Est. expirySep 13, 2033(~7.1 yrs left)· nominal 20-yr term from priority
C12Q 1/6874C12Q 1/6806
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Claims
Abstract
An aqueous reagent solution includes a magnesium salt in a range of 30 mM to 500 mM, a potassium salt in a range of 0.8 M to 1.0 M, a buffering agent in a range of 150 mM to 500 mM, and a surfactant in a range of 0.05% to 0.5%.
Claims
exact text as granted — not AI-modifiedWhat is claimed is:
1 . An aqueous reagent solution comprising:
a magnesium salt in a range of 30 mM to 500 mM; a potassium salt in a range of 0.8 M to 1.0 M; a buffering agent in a range of 150 mM to 500 mM; and a surfactant in a range of 0.05% to 0.5%.
2 . The aqueous reagent solution of claim 1 , wherein the pH is in a range of 7 to 9.
3 . The aqueous reagent solution of claim 1 , wherein the buffering agent includes tris(hydroxymethyl)aminomethane.
4 . The aqueous reagent solution of claim 1 , wherein the surfactant includes a polyethylene glycol based surfactant.
5 . The aqueous reagent solution of claim 4 , wherein the polyethylene glycol based surfactant includes an octylphenyl ether of polyethylene glycol.
6 . The aqueous reagent solution of claim 1 , further comprising polyethylene glycol based polymer
7 . The aqueous reagent solution of claim 6 , wherein the polyethylene glycol based surfactant has a molecular weight in a range of 1000 Da to 100000 Da.
8 . The aqueous reagent solution of claim 7 , wherein the polyethylene glycol based surfactant has a molecular weight in a range of 2000 Da to 20000 Da.
9 . The aqueous reagent solution of claim 1 , wherein the polyethylene glycol based polymer is present in a range of 0.1 wt % to 20.0 wt %.
10 . The aqueous reagent solution of claim 9 , wherein the polyethylene glycol based polymer is present in a range of 0.5 wt % to 15.0 wt %.
11 . The aqueous reagent solution of claim 1 , wherein the magnesium salt is present in a range of 50 mM to 1M.
12 . The aqueous reagent solution of claim 11 , wherein the magnesium salt is present in a range of 50 mM to 800 mM.
13 . An aqueous reagent solution comprising:
a magnesium salt in a range of 30 mM to 1M; polyethylene glycol based polymer in a range of 0.5 wt % to 10.0 wt %; a potassium salt in a range of 0.8 M to 1.0 M; and a buffering agent in a range of 150 mM to 500 mM.
14 . The aqueous reagent solution of claim 13 , wherein the pH is in a range of 7 to 9.
15 . The aqueous reagent solution of claim 13 , wherein the magnesium salt is present in a range of 50 mM to 1M.
16 . The aqueous reagent solution of claim 15 , wherein the magnesium salt is present in a range of 50 mM to 800 mM.
17 . The aqueous reagent solution of claim 13 , wherein the polyethylene glycol based surfactant has a molecular weight in a range of 1000 Da to 100000 Da.
18 . The aqueous reagent solution of claim 17 , wherein the polyethylene glycol based surfactant has a molecular weight in a range of 2000 Da to 20000 Da.
19 . The aqueous reagent solution of claim 13 , wherein the polyethylene glycol based polymer is present in a range of 0.1 wt % to 20.0 wt %.
20 . The aqueous reagent solution of claim 19 , wherein the polyethylene glycol based polymer is present in a range of 0.5 wt % to 15.0 wt %.Join the waitlist — get patent alerts
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