Apparatus, kits and methods for the prediction of onset of sepsis

Abstract

The present invention provides kits, methods, and apparatus for analysing a biological sample from an animal to predict (pre-symptomatically) and monitor the development of sepsis, utilising biomarker signatures, and especially biomarker signatures capable of providing a mean predictive accuracy of at least 92% to differentiate development of sepsis from non-sepsis.

1 . A diagnostic kit for predicting development of sepsis prior to onset of symptoms, comprising reagents for detecting nucleic acid gene products or expression levels of members of a biomarker signature consisting of 266 genes of Table I in a sample obtained from a patient at a risk of sepsis,
 wherein the reagents comprise one or more of fluorescently labelled oligonucleotide probes or fluorescently labeled primers,   wherein the fluorescently labelled oligonucleotide probes or fluorescently labeled primers consist of probes and primers each capable of specific binding and detection of gene products of at least 25 of the members of the biomarker signature,   and wherein the at least 25 of the members of the biomarker signature comprise C16ORF7, C5ORF39, C9ORF103, CD177, FCER1A, GAS7, LOC285176, MYBPC3, NDST2, EBI2, RPL13A, RPL18A, RPL32, RPL36, RPL9, RPS20, RPS29, RPS6, SIGIRR, TCEA3, TCTN1, TIMM9, TOMM7, ZFAND1 and ZNHIT3.   
     
     
         2 . The kit of  claim 1 , wherein the nucleic acid gene products are transcribed ribonucleic acids or cDNA. 
     
     
         3 . The kit of  claim 1 , wherein the patient is a post-surgical patient, an immunocompromised individual, an intensive-care patient or a burn patient. 
     
     
         4 . The kit of  claim 1 , wherein the at least 25 of the members of the biomarker signature further comprise ATP9A, CACNA1E, DHRS3, EEF1B2, FLT3LG, GRB10, HLA.DMA, HS.445036, IL1R1, IL1R2, NCOA3, RPL10A, LOC646483, RPL18, SLBP, SLC26A6, SMPDL3A, SORBS3, THBS3 and THNSL1. 
     
     
         5 . The kit of  claim 1 , wherein the at least 25 of the members of the biomarker signature consist of all 266 genes of Table 1. 
     
     
         6 . The kit of  claim 1 , wherein the reagents comprise the fluorescently labelled oligonucleotide probes. 
     
     
         7 . The kit of  claim 1 , wherein the reagents comprise the fluorescently labelled primers. 
     
     
         8 . A system for analysis of a biological sample obtained from a patient at risk of sepsis to predict or monitor development of sepsis in the patient, the system comprising:
 a detector for monitoring, measuring or detecting the nucleic acid gene products or the expression levels of the members of a biomarker signature;   the kit of  claim 1 ;   and a computer processor configured to analyze data produced by the detector, and to provide an output.   
     
     
         9 . A diagnostic kit for predicting development of sepsis prior to onset of symptoms, comprising reagents for detecting gene products or expression levels of members of a biomarker signature in a sample obtained from a patient at a risk of sepsis,
 wherein the biomarker signature consists of 266 genes of Table I,   wherein the reagents comprise a microarray with immobilized probes, the probes consisting of probes each suitable for specific binding to and detection of gene products of at least 25 of the members of the biomarker signature,   and wherein the at least 25 of the members of the biomarker signature comprise C16ORF7, C5ORF39, C9ORF103, CD177, FCER1A, GAS7, LOC285176, MYBPC3, NDST2, EBI2, RPL13A, RPL18A, RPL32, RPL36, RPL9, RPS20, RPS29, RPS6, SIGIRR, TCEA3, TCTN1, TIMM9, TOMM7, ZFAND1 and ZNHIT3.   
     
     
         10 . The kit of  claim 9 , wherein the gene products are nucleic acids. 
     
     
         11 . The kit of  claim 10 , wherein the nucleic acids are transcribed ribonucleic acids or cDNA. 
     
     
         12 . The kit of  claim 9 , wherein the gene products are proteins. 
     
     
         13 . The kit of  claim 9 , wherein the patient is a post-surgical patient, an immunocompromised individual, an intensive-care patient or a burn patient. 
     
     
         14 . The kit of  claim 9 , wherein the at least 25 of the members of the biomarker signature further comprise ATP9A, CACNA1E, DHRS3, EEF1B2, FLT3LG, GRB10, HLA.DMA, HS.445036, IL1R1, IL1R2, NCOA3, RPL10A, LOC646483, RPL18, SLBP, SLC26A6, SMPDL3A, SORBS3, THBS3 and THNSL1. 
     
     
         15 . The kit of  claim 9 , wherein the at least 25 of the members of the biomarker signature consist of all 266 genes of Table 1. 
     
     
         16 . A system for analysis of a biological sample obtained from a patient at risk of developing sepsis to predict or monitor development of sepsis in the patient, the system comprising:
 a detector for monitoring, measuring or detecting the gene products or the expression levels of the member of a biomarker signature;   the kit of  claim 9 ;   and a computer processor configured to analyze data produced by the detector, and to provide an output.