Methods and kits for reducing adapter-dimer formation

Abstract

The current teachings relate to methods for reducing adapter-dimer formation, particularly when preparing nucleic acids of interest for subsequent amplification and/or sequencing. Also described are kits for use in performing certain disclosed methods.

1 . A kit comprising at least one 3′ adapter comprising 1-25 randomized bases on the 5′ end, at least one oligonucleotide complementary to at least a portion of the 3′ adapter, and at least one 5′ adapter with 1-25 randomized bases on the 3′ end. 
     
     
         2 . The kit of  claim 1  further comprising a DNA polymerase, at least one ligase, or a DNA polymerase and at least one ligase. 
     
     
         3 . The kit of  claim 2 , wherein the at least one ligase comprises at least one of: T4 RNA ligase 2, T4 RNA ligase 1, or  Methanobacterium thermoautotrophicum  RNA ligase.