Methods of assaying nucleic acids of low quantities using a buffer nucleic acid

Abstract

This disclosure provides methods, kits, and compositions to amplify sample DNA of low quantity by adding an amount of buffer DNA before amplification. The method protects the sample DNA against degradation and PCR bias. The method disclosed herein are useful to amplify DNA of low amount, such as genomic DNA from a single cell and therefore are especially useful for genetic diagnostic tests and precision medicine.

We claim: 
     
         1 . A method of amplifying a sample RNA comprising the steps of:
 a. converting the sample RNA into a sample cDNA,   b. mixing a buffer DNA with the sample cDNA to form a total assay DNA; and   d. amplifying the total assay DNA.   
     
     
         2 . The method of  claim 1 , wherein the sample RNA is from a single cell without amplification. 
     
     
         3 . A kit for treating a sample DNA comprising:
 a. a buffer DNA   wherein the buffer DNA is at least 40 bp, and in an amount that when mixed with the sample DNA, the mass ratio of the buffer DNA and the sample DNA is at least 10,   wherein each molecule of the buffer DNA comprises at least one or more rare cleavage sites, and   b instruction for mixing buffer DNA and a sample DNA.   
     
     
         4 . The kit of  claim 3 , further comprise a polymerase, one or more primers for amplifying the sample DNA. 
     
     
         5 . The kit of  claim 3 , wherein the kit further comprises adaptors and a ligase for ligating the adaptors to the sample DNA and buffer DNA.