Device, system, and method including micro-patterned cell treatment array

Abstract

Devices, systems, or methods are disclosed herein for treatment of disease in a vertebrate subject. The device can include a quasi-planar substrate; and one or more laterally-mobile effector molecule types at least partially embedded within the quasi-planar substrate, wherein the one or more laterally-mobile effector molecule types is configured to interact with one or more cell types. The device can further include one or more sensors configured to detect at least one aspect of an interaction between the at least one of the one or more laterally-mobile effector molecule types and the one or more cell types; and a controller in communication with the one or more sensors, wherein the controller is configured to responsively initiate modification of at least one of the one or more laterally-mobile effector molecule types, the quasi-planar substrate, and the one or more cell types.

What is claimed is: 
     
         1 .- 152 . (canceled) 
     
     
         153 . A microfluidic chip comprising:
 a fluid circuit including a plurality of channels, wherein the channels include a quasi-planar substrate having one or more laterally-mobile effector molecule types at least partially embedded within the quasi-planar substrate;   at least one sensor affixed to the microfluidic chip;   at least one microfluidic pump affixed to the fluid circuit; and   a controller operably connected to the at least one sensor and the at least one microfluidic pump.   
     
     
         154 . The microfluidic chip of  claim 153 , wherein the fluid circuit is part of a micropattern array including a plurality of fluid circuits. 
     
     
         155 . The microfluidic chip of  claim 153 ; wherein the fluid circuit comprises:
 interconnected channels; and   single cell bays.   
     
     
         156 . The microfluidic chip of  claim 153 , wherein the fluid circuit comprises:
 a first channel;   a first cell bay connected to the first channel;   a second channel;   a second cell bay connected to the second channel; and   a cell collection trap.   
     
     
         157 . The microfluidic chip of  claim 153 ; wherein the quasi-planar substrate comprises:
 a first region including a first laterally-mobile effector molecule type; and   a second region including a second laterally-mobile effector molecule type.   
     
     
         158 . The microfluidic chip of  claim 153 , wherein at least one of the one or more laterally-mobile effector molecule types is configured to interact with one or more cell types. 
     
     
         159 . The microfluidic chip of  claim 153 , wherein at least one of the one or more laterally-mobile effector molecule types comprises:
 a ligand molecule, a receptor molecule, or a cytokine.   
     
     
         160 . The microfluidic chip of  claim 153 , wherein at least one of the one or more laterally-mobile effector molecule types comprises:
 at least one of an integral membrane protein, glycophosphatidylinositol-anchored protein, fusion protein, conjugated protein, aptamer or polymer.   
     
     
         161 . The microfluidic chip of  claim 153 , wherein the at least one sensor comprises:
 at least one of a thermal detector, an electrical detector, a chemical detector, an optical detector, an ion detector, a biological detector, a radioisotope detector, an electrochemical detector, a radiation detector, an acoustic detector, a magnetic detector, a capacitive detector, a pressure detector, an ultrasonic detector, an infrared detector, a microwave motion detector, a radar detector, an electric eye, or an image sensor.   
     
     
         162 . The microfluidic chip of  claim 153 , wherein the at least one sensor is configured to detect a location of a portion of the one or more laterally-mobile effector molecule types. 
     
     
         163 . The microfluidic chip of  claim 153 , wherein the at least one microfluidic pump affixed to the fluid circuit is configured to vary fluid flow pressure within the fluid circuit in response to a signal from the controller. 
     
     
         164 . The microfluidic chip of  claim 153 , wherein the controller is configured to initiate modification of at least one of the one or more laterally-mobile effector molecule types, the quasi-planar substrate, and the one or more cell types. 
     
     
         165 . The microfluidic chip of  claim 153 , wherein the controller is configured to send a control signal to the at least one microfluidic pump in response to signals received from the sensor. 
     
     
         166 . The microfluidic chip of  claim 153 , comprising:
 at least one heating element.   
     
     
         167 . A method of separating cell types, comprising:
 receiving a fluid sample into a fluidic circuit including one or more laterally-mobile effector molecule types at least partially embedded within a quasi-planar substrate;   pumping the fluid sample through channels within the circuit;   sensing interaction of cells from the fluid sample with at least one of the one or more laterally-mobile effector molecule types;   sending a signal to a controller of the sensed interaction;   sending a signal from the controller to a fluid pump in response to the signal from the sensor.   
     
     
         168 . The method of  claim 167 , wherein the fluid sample includes:
 dendritic cells derived from peripheral blood.   
     
     
         169 . The method of  claim 167 , wherein the fluid sample includes:
 cultured immune system cells.   
     
     
         170 . The method of  claim 167 , wherein the fluidic circuit is part of a micropatterned array. 
     
     
         171 . The method of  claim 167 , wherein the one or more laterally-mobile effector molecule types is embedded in a lipid bilayer. 
     
     
         172 . The method of  claim 167 , wherein the sensing interaction includes optical sensing. 
     
     
         173 . The method of  claim 167 , wherein the sensing interaction includes binding of one or more aptamer types to the at least one of the one or more laterally-mobile effector molecule types. 
     
     
         174 . The method of  claim 167 , wherein the sensing interaction results in sending an electronic signal from the sensor. 
     
     
         175 . The method of  claim 167 , further comprising:
 pumping the fluid sample with the fluid pump though channels within the circuit in response to received signals from the controller.   
     
     
         176 . The method of  claim 167 , further comprising:
 maintaining a temperature of the fluidic circuit with a heating element.