Novel graft polymer, temperature-responsive substrate for cell culture using the same and production method therefor, as well as liquid chromatographic carrier having the novel graft polymer immomibilized thereon and liquid chromatographic method using the same

Abstract

By using a graft polymer comprising a dendritic polymer with a styrene skeleton and a hydrophilic polymer grafted to a terminal thereof, a temperature-responsive substrate for cell culture having a temperature-responsive surface for cell culture that allows cells to be cultured with high efficiency and which yet allows cultured cells to be exfoliated in a short period of time and with high efficiency by simply changing the temperature of the substrate surface can be prepared conveniently. If this temperature-responsive substrate for cell culture is used, cells obtained from a variety of tissues can be cultured with high efficiency. If this culture method is utilized, cultured cells can be exfoliated intact in a short amount of time with high efficiency. In addition, by using this graft polymer, a wide range of peptides and proteins can also be separated by simply changing the temperature of a chromatographic carrier. This allows for convenient separation procedure and improves the efficiency of separating operations. What is more, the stereoregularity of the dendritic polymer per se may be utilized to enable separation of solutes based on differences in their molecular structures.

1 . A graft polymer comprising a dendritic polymer with a styrene skeleton and a hydrophilic polymer grafted at a terminal thereof. 
     
     
         2 . The graft polymer according to  claim 1 , which has electric charges at a terminal of the dendritic polymer. 
     
     
         3 . The graft polymer according to  claim 1 , wherein the hydrophilic polymer is one having temperature response. 
     
     
         4 . The graft polymer according to  claim 3 , wherein the polymer having temperature response comprises any one or more of a poly-N-substituted acrylamide derivative, a poly-N-substituted methacrylamide derivative, a copolymer thereof, polyvinyl methyl ether, and partially acetylated polyvinyl alcohol, or a copolymer thereof with another monomer. 
     
     
         5 . The graft polymer according to  claim 4 , wherein the polymer having temperature response is poly-N-isopropylacrylamide. 
     
     
         6 . The graft polymer according to  claim 4 , wherein the another monomer is a monomer having electric charges and/or a hydrophobic monomer. 
     
     
         7 . The graft polymer according to  claim 1 , wherein the hydrophilic polymer comprises any one or more of polyacrylamide, poly-N,N-diethylacrylamide, poly-N,N-dimethylacrylamide, acrylate having polyethylene oxide in side chains, and methacrylate having polyethylene oxide in side chains. 
     
     
         8 . The graft polymer according to  claim 1 , wherein the hydrophilic polymer is a mixture of the polymers having temperature response comprising any one or more of a poly-N-substituted acrylamide derivative, a poly-N-substituted methacrylamide derivative, a copolymer thereof, polyvinyl methyl ether, and partially acetylated polyvinyl alcohol, or a copolymer thereof with another monomer.