Method and materials for isolation of nucleic acid materials

Abstract

A method for nucleic acid isolation comprising: receiving a binding moiety solution within a process chamber; mixing the binding moiety solution with a biological sample, within the process chamber, in order to produce a moiety-sample mixture; incubating the moiety-sample mixture during a time window, thereby producing a solution comprising a set of moiety-bound nucleic acid particles and a waste volume; separating the set of moiety-bound nucleic acid particles from the waste volume; washing the set of moiety-bound nucleic acid particles; and releasing a nucleic acid sample from the set of moiety-bound nucleic acid particles. The method preferably utilizes a binding moiety comprising at least one of poly(allylamine) and polypropylenimine tetramine dendrimer, both of which reversibly bind and unbind to nucleic acids based upon environmental pH.

We claim: 
     
         1 . A method for nucleic acid isolation, comprising:
 receiving a biological sample;   receiving affinity moiety-coated microparticles comprising magnetic microparticles bonded to a moiety comprising at least one of Poly(allylamine) (PAA) and Polypropylenimine tetramine dendrimer (DABAM);   incubating the affinity moiety-coated microparticles with the biological sample for reversibly binding the moiety to nucleic acid material of the biological sample, thereby producing moiety-bound nucleic acid particles within a moiety-sample mixture;   separating the moiety-bound nucleic acid particles from the moiety-sample mixture; and   releasing the nucleic acid material from the moiety of the moiety-bound nucleic acid particles.