Apparatus and method for isolating stem cells

Abstract

An apparatus for isolating stems cells from extracted mammalian tissue comprising: a portable hollow casing having fixed dimensions and a sized internal spatial volume; a filter housed and contained within said sized internal spatial volume, wherein said filter captures particles in said extracted mammalian tissue having a diameter of about 5 to 10 microns or more and allows particles in said extracted mammalian tissue having a diameter of less than about 5 to 10 microns to pass through; a first channel to which a container holding said extracted mammalian tissue can attach, and through which said extracted mammalian tissue is input into the hollow casing; wherein a stem cell collection chamber can attach to said first channel, and the particles having a diameter of about 5 to 10 microns or more are output from the hollow casing through said first channel and collected in the stem cell collection chamber; and a second channel to which a remnant collection chamber can attach, and through which the particles having a diameter of less than about 5 to 10 microns are output from the hollow casing and collected in the remnant collection chamber.

1 . An apparatus for isolating stems cells from extracted mammalian tissue comprising:
 a portable hollow casing having fixed dimensions and a sized internal spatial volume;   a filter housed and contained within said sized internal spatial volume, wherein said filter captures particles in said extracted mammalian tissue having a diameter of about 5 to 10 microns or more and allows particles in said extracted mammalian tissue having a diameter of less than about 5 to 10 microns to pass through;   a first channel to which a container holding said extracted mammalian tissue can attach, and through which said extracted mammalian tissue is input into the hollow casing;   wherein a stem cell collection chamber can attach to said first channel, and the particles having a diameter of about 5 to 10 microns or more are output from the hollow casing through said first channel and collected in the stem cell collection chamber; and   a second channel to which a remnant collection chamber can attach, and through which the particles having a diameter of less than about 5 to 10 microns are output from the hollow casing and collected in the remnant collection chamber.   
     
     
         2 . The apparatus of  claim 1 , wherein the hollow casing comprises a top portion and a bottom portion which are detachable from each other;
 wherein the filter is inserted between the top portion and the bottom portion.   
     
     
         3 . The apparatus of  claim 2 , wherein a sample of blood or blood plasma is input into the bottom portion of the hollow casing. 
     
     
         4 . The apparatus of  claim 3 , wherein the sample of blood or blood plasma is input into the bottom portion of the hollow casing prior to the extracted mammalian tissue being input into the hollow casing;
 wherein the sample of blood or blood plasma is input into the bottom portion of the hollow casing via the second channel   
     
     
         5 . The apparatus of  claim 2 , wherein a saline solution is input into the bottom portion of the hollow casing. 
     
     
         6 . The apparatus of  claim 5 , wherein the saline solution has a salt concentration above 0.9%;
 wherein the saline solution is input into the bottom portion of the hollow casing prior to the extracted mammalian tissue being input into the hollow casing;   wherein the saline solution is input into the bottom portion of the hollow casing via the second channel.   
     
     
         7 . The apparatus of  claim 1 , wherein the filter comprises pores that allow particles having a diameter of less than about 5 microns to pass through. 
     
     
         8 . The apparatus of  claim 1 , wherein the container holding the extracted mammalian tissue is a barrel of an extraction syringe;
 wherein depressing a plunger of the extraction syringe causes the extracted mammalian tissue to be expelled out an opening of the barrel, through the first channel and into the sized internal spatial volume of the hollow casing.   
     
     
         9 . The apparatus of  claim 1 , wherein the stem cell collection chamber is a barrel of a stem cell collection syringe;
 wherein pulling a plunger of the stem cell collection syringe causes the particles in said extracted mammalian tissue having a diameter of about 5 to 10 microns or more to be aspirated from the hollow casing, through the first channel and an opening in the barrel of the stem cell collection syringe, and into the barrel of the stem cell collection syringe.   
     
     
         10 . The apparatus of  claim 1 , wherein the remnant collection chamber is a barrel of a remnant collection syringe;
 wherein pulling a plunger of the remnant collection syringe causes the particles in said extracted mammalian tissue having a diameter of less than about 5 to 10 microns to be aspirated from the hollow casing, through the second channel and an opening in the barrel of the remnant collection syringe, and into the barrel of the remnant collection syringe.   
     
     
         11 . A method for isolating stem cells from extracted mammalian tissue comprising:
 (a) providing a container holding said extracted mammalian tissue;   (b) attaching the container holding said extracted mammalian tissue to an apparatus for isolating stems cells from extracted mammalian tissue comprising:   a portable hollow casing having fixed dimensions and a sized internal spatial volume;   a filter housed and contained within said sized internal spatial volume, wherein said filter captures particles in said extracted mammalian tissue having a diameter of about 5 to 10 microns or more and allows particles in said extracted mammalian tissue having a diameter of less than about 5 to 10 microns to pass through;   a first channel to which the container holding said extracted mammalian tissue can attach, and through which said extracted mammalian tissue is input into the hollow casing;   wherein a stem cell collection chamber can attach to said first channel, and the particles having a diameter of about 5 to 10 microns or more are output from the hollow casing through said first channel and collected in the stem cell collection chamber; and   a second channel to which a remnant collection chamber can attach, and through which the particles having a diameter of less than about 5 to 10 microns are output from the hollow casing and collected in the remnant collection chamber;   wherein the hollow casing comprises a top portion and a bottom portion which are detachable from each other;   wherein the filter is inserted between the top portion and the bottom portion;   (c) causing the extracted mammalian tissue to move from the container holding said extracted mammalian tissue, through the first channel, into the hollow casing and into contact with the filter;   (d) allowing particles having a diameter of about 5 to 10 microns or more to be captured by the filter, move out of the hollow casing through the first channel and into the stem cell collection chamber; and   (e) allowing particles having a diameter of less than about 5 to 10 microns to pass through the filter, move out of the hollow casing through the second channel and into the remnant collection chamber.   
     
     
         12 . The method of  claim 11 , wherein a sample of blood or blood plasma is input into the bottom portion of the hollow casing. 
     
     
         13 . The method of  claim 12 , wherein the sample of blood or blood plasma is input into the bottom portion of the hollow casing prior to the extracted mammalian tissue being input into the hollow casing. 
     
     
         14 . The method of  claim 12 , wherein the sample of blood or blood plasma is taken from a common subject as the extracted mammalian tissue. 
     
     
         15 . The method of  claim 12 , wherein the sample of blood or blood plasma is input into the bottom portion of the hollow casing via the second channel 
     
     
         16 . The method of  claim 11 , wherein a saline solution is input into the bottom portion of the hollow casing. 
     
     
         17 . The method of  claim 16 , wherein the saline solution has a salt concentration above 0.9%. 
     
     
         18 . The method of  claim 16 , wherein the saline solution has a salt concentration of about 2% to 23%. 
     
     
         19 . The method of  claim 16 , wherein the saline solution is input into the bottom portion of the hollow casing prior to the extracted mammalian tissue being input into the hollow casing. 
     
     
         20 . The method of  claim 16 , wherein the saline solution is input into the bottom portion of the hollow casing via the second channel.