Automated priming and library loading device

Abstract

Provided herein are automated apparatus for the identification of microorganisms in various samples. The disclosure solves existing challenges encountered in identifying and distinguishing various types of microorganisms, including viruses and bacteria in a timely, efficient, and automated manner by sequencing.

What is claimed is: 
     
         1 . A nucleic acid sequencing apparatus comprising:
 (a) a nucleic acid library preparation compartment comprising two or more chambers configured to prepare a plurality of nucleic acids from a sample for a sequencing reaction;   (b) a nucleic acid sequencing chamber, wherein said nucleic acid sequencing chamber comprises one or more flow cells comprising a plurality of pores or sequencing cartridges configured for the passage of a nucleic acid strand, wherein said nucleic acid library preparation compartment is operatively connected to said nucleic acid sequencing chamber; and   (c) an automated platform, wherein said automated platform is programmed to robotically move a sample from said nucleic acid library preparation compartment into said nucleic acid sequencing chamber.   
     
     
         2 . The nucleic acid sequencing apparatus of  claim 1 , wherein said automated platform moves a second sample from said nucleic acid library preparation compartment into said nucleic acid sequencing chamber upon detecting a failure of a sequencing reaction. 
     
     
         3 . The nucleic acid sequencing apparatus of  claim 1 , wherein said automated platform moves a second sample from said nucleic acid library preparation compartment into said nucleic acid sequencing chamber upon detecting a completion of a sequencing reaction. 
     
     
         4 . The nucleic acid sequencing apparatus of  claim 1 , further comprising adding a barcode to a plurality of nucleic acids in said two or more chambers of (a), thereby providing a plurality of barcoded nucleic acids for said sequencing reaction. 
     
     
         5 . The nucleic acid sequencing apparatus of  claim 1 , further comprising adding a plurality of mutually exclusive barcodes to a plurality of nucleic acids in said two or more chambers of (a), thereby providing a plurality of mutually exclusive barcoded nucleic acids. 
     
     
         6 . The nucleic acid sequencing apparatus of  claim 5 , wherein said automated platform robotically moves two or more of said mutually exclusive barcoded nucleic acids into said nucleic acid sequencing chamber. 
     
     
         7 . The nucleic acid sequencing apparatus of  claim 5 , wherein said automated platform robotically moves two or more of said mutually exclusive barcoded nucleic acids into a same flow cell of said one or more flow cells. 
     
     
         8 . The nucleic acid sequencing apparatus of  claim 5 , wherein said sample is a food or an environmental sample. 
     
     
         9 . The nucleic acid sequencing apparatus of  claim 5 , wherein said sample is a non-food sample. 
     
     
         10 . The nucleic acid sequencing apparatus of  claim 9 , wherein said sample comprise blood, plasma, urine, tissue, feces, bone marrow, saliva or cerebrospinal fluid. 
     
     
         11 . The nucleic acid sequencing apparatus of  claim 1 , wherein the apparatus comprises two or more flow cells. 
     
     
         12 . The nucleic acid sequencing apparatus of  claim 11 , wherein one or more of the flow cells are juxtaposed to one another. 
     
     
         13 . The nucleic acid apparatus of  claim 1 , wherein said automated platform is programmed to flush at least one of said one or more flow cells. 
     
     
         14 . The nucleic acid apparatus of  claim 1 , wherein said automated platform is programmed to move a liquid sample of about 0.1 μl to about 1000 μl from said nucleic acid library preparation compartment into said sample input port. 
     
     
         15 . The nucleic acid apparatus of  claim 1 , wherein said plurality of nucleic acid comprises a nucleic acid from a microorganism. 
     
     
         16 . The nucleic acid apparatus of  claim 15 , wherein said microorganism is a bacteria, a fungus, a parasite, a protozoa, or a virus. 
     
     
         17 . The nucleic acid apparatus of  claim 16 , wherein said microorganism is a virus. 
     
     
         18 . The nucleic acid apparatus of  claim 16 , wherein said microorganism is a bacteria. 
     
     
         19 . The nucleic acid apparatus of  claim 1 , wherein said sequencing reaction is a nanopore sequencing. 
     
     
         20 . The nucleic acid apparatus of  claim 1 , wherein said sequencing reaction is a sequencing-by-synthesis reaction.