Method for preparing microbial preparation and microbial preparation produced by the same

Abstract

The present disclosure relates to a method for preparing an aglycone or hydrolyzed glycoside converted from a glycoside and, specifically, to a method for preparing an aglycone or hydrolyzed glycoside from a glycoside by converting a glycoside into an aglycone form or hydrolyzed glycoside by using a microorganism producing β-glycosidase, and then recovering the aglycone or hydrolyzed glycoside accumulated in the cells of the microorganism.

What is claimed is: 
     
         1 . A microorganism or a lysate thereof, wherein the microorganism is a β-glycosidase-expressing microorganism and contains intracellularly accumulated converted ginsenoside or hydrolyzed glycoside. 
     
     
         2 . The microorganism or lysate of  claim 1 , wherein said intracellularly accumulated converted ginsenoside or hydrolyzed glycoside comprises one selected from the group consisting of rg3, rc and f2. 
     
     
         3 . The microorganism or lysate of  claim 1 , wherein said intracellularly accumulated converted ginsenoside or hydrolyzed glycoside comprises one  ginseng  extracts comprise one or more of a ginsenoside selected from the group consisting of rg1 and rb1. 
     
     
         4 . The microorganism or lysate of  claim 1 , wherein said intracellularly accumulated converted ginsenoside or hydrolyzed glycoside comprises one or more selected from the group consisting of genistein, daidzein, glycitein, sapogenin, 3,4-Hydroxyphenylactic acid, 4-HPA, m-coumaric acid, p-coumaric acid, O-beta-D-glucuroniodes, stilbenoids, rutin, quercetin, hesparetin, baicalein, wogonin, mogroside IIIE, mendelonitrile, benzaldehyde, and coumarin-derived compounds. 
     
     
         5 . The microorganism or lysate of  claim 1 , wherein said intracellularly accumulated converted ginsenoside or hydrolyzed glycoside comprises one or more of rg3, rc, and f2. 
     
     
         6 . The microorganism or lysate of  claim 1 , wherein the microorganism comprises one or more selected from the group consisting of lactic acid bacteria,  bifidus , yeast,  corynebacterium, aspergillus  and  clostridium.    
     
     
         7 . The microorganism or lysate of  claim 1 , wherein the microorganism comprises one or more selected from the group consisting of generally recognized as safe (GRAS) microorganisms,  bifidus , yeast,  Bacillus licheniformis, S. thermophilus, L. casei, Streptomyces  sp.  Bifidobacteria, Lactobacillus delbrueckii  Rh2 , Sporosarcina  sp.,  Saccharomyces cerevisiae, Pyrococcus furiosus, Lactobacillus plantarum, Aspergillus ochraceus, Aactobacillus delbrueckii  Rh2,  Pseudomonas  sp.,  Aspergillus niger; Pseudomonas fluorescens, Bifidobacterium adolescentis, Aspergillus sojae, Cunninghamella blakesleeana, Cifidobacteria  and  Lactobacillus , Lactic acid bacteria,  Bifidobacterium pseudocatenulatum, Penicillium melinii, Eubacterium ramulus, Clostridium orbiscindens, Aspergillus awamori, Lactobacillus brevis, Aspergillus parasiticus speare  BGB,  Aspergillus aculeatus , and  Aspergillus niger.    
     
     
         8 . The microorganism or lysate of  claim 1 , wherein the microorganism comprises a lactic acid bacterium. 
     
     
         9 . The microorganism or lysate of  claim 1 , wherein the microorganism comprises one or more selected from the group consisting of  L. plantarum, L. sakei, L. rhamnosus  GG;  L. delbrueckii, L. acidophilus, L. johnsonii, L. casei  and  L. gasseri.    
     
     
         10 . The microorganism or lysate of  claim 1 , wherein the microorganism comprises  Lactobacillus plantarum  K8 (accession number: KCTC 10887BP). 
     
     
         11 . A lysate of the microorganism of  claim 1 . 
     
     
         12 . An antioxidant, intestinal regulation, cosmetic, probiotic, or food composition, comprising the microorganism or lysate of  claim 1 . 
     
     
         13 . A cosmetic composition comprising the microorganism or lysate of  claim 1  for enhancement of skin function of skin moisturizing, skin color, skin elasticity, wrinkles or dermis density. 
     
     
         14 . A probiotic composition comprising the microorganism of  claim 1 , in a dose form with a viable cell count in a range of from 10 8  to 10 10  CFU/day.