Resistance to heterodera carotae and methods for use

Abstract

The present application relates to the use of Daucus carota plants which do not belong to the Daucus carota subsp. sativus subspecies of cultured carrots to disinfect a culture medium infested with Heterodera carotae nematodes, using the nematicidal power of the plants. The present application also relates to Daucus carota plants which are resistant to the Heterodera carotae nematode, and to methods for culturing such plants which make it possible to reduce the population of Heterodera carotae nematodes present in a culture medium.

1 - 7 . (canceled) 
     
     
         8 . A method for reducing the population of  Heterodera carotae  nematodes in cultivation medium infested with such nematodes, comprising cultivating  Daucus carota  plants which do not belong to the sub-species of cultivated  Daucus carota  subsp.  sativus  carrots, wherein said plants have a nematicidal effect on said nematodes. 
     
     
         9 . The method according to  claim 8 , wherein said reduction in the population of nematodes involves a reduction in the number of cysts and/or J2 juveniles. 
     
     
         10 . The method according to  claim 8 , wherein said plants are obtained from seeds deposited with the NCIMB under the number NCIMB 42351. 
     
     
         11 . The method according to  claim 8 , wherein said cultivating is for at least 4 months. 
     
     
         12 . The method according to  claim 8 , wherein the infested cultivation medium is earth or sand in an open field. 
     
     
         13 . The method according to  claim 8 , wherein the level of infestation of the cultivation medium is reduced to less than one J2 female  Heterodera carotae  larva per gram of medium. 
     
     
         14 . The method according to  claim 8 , wherein the population of  Heterodera carotae  nematodes in the cultivation medium is reduced by at least 40%. 
     
     
         15 . The method according to  claim 8 , wherein the number of cysts or female nematodes in the cultivation medium is reduced by at least 50%. 
     
     
         16 . The method according to  claim 8 , wherein said cultivating is carried out at a density of 0.8 to 1.4 million seeds per hectare. 
     
     
         17 . A population of plants or seeds of  Daucus carota  plants, obtained or derived from seeds deposited under the accession number NCIMB 42351, wherein said plants have a nematicidal effect on a population of  Heterodera carotae  nematodes. 
     
     
         18 . The population of plants or plant seeds according to  claim 17 , wherein the nematicidal effect involves (a) a reduction in the number of J2 juveniles, (b) a reduction in the number of cysts, (c) a masculinization of more than 50% of the larvae of the  Heterodera carotae  nematode, or (d) combinations of (a), (b) and (c), after a cultivation period of at least 3 months. 
     
     
         19 . The method of  claim 11  wherein said cultivation period is at least 5 months. 
     
     
         20 . The method of  claim 11  wherein said cultivation period is at least 6 months. 
     
     
         21 . The method of  claim 14 , wherein the population of  Heterodera carotae  nematodes in the cultivation medium is reduced by at least 50%. 
     
     
         22 . The method of  claim 14 , wherein the population of  Heterodera carotae  nematodes in the cultivation medium is reduced by at least 60%. 
     
     
         23 . The method of  claim 14 , wherein the population of  Heterodera carotae  nematodes in the cultivation medium is reduced by at least 75%. 
     
     
         24 . The population of plants or plant seeds according to  claim 17 , wherein said plant or plant seeds are obtained by propagation or crossing and selection of plants originating from seeds deposited under the accessions number NCIMB 42351. 
     
     
         25 . A method for disinfecting a soil contaminated by the carrot cyst nematode  Heterodera carotae , comprising cultivating in said contaminated soil  Daucus carota  plants resistant to said cyst nematode and which do not belong to the sub-species of cultivated  Daucus carota  subsp.  sativus  carrots, so that to restrict the multiplication of the nematode thereby disinfecting the soil.