Method of myceliating coffee

Abstract

The present invention includes a method of myceliating coffee beans including sterilizing the coffee beans, preparing a liquid tissue culture including an aliquot of fungal liquid tissue culture derived from liquid state fermentation, and inoculating the coffee beans with the fungal liquid tissue culture. Next, the step of enabling mycelium growth on the coffee includes controlling temperature, humidity, and sterility of the environment. The aliquot of fungal liquid tissue culture is optionally agitated to form hyphael conglomerations of mycelium having sizes of less than 1 mm in diameter.

What is claimed is: 
     
         1 . A method for the preparation of a myceliated agricultural substrate, comprising:
 sterilizing the agricultural substrate;   providing a fungal liquid tissue culture, wherein the fungal culture is selected from the group consisting of  Lentinula edodes, Pleurotus ostreatus, Morchella hortensis,  and golden  Morchella;      inoculating the sterilized agricultural substrate with the fungal liquid tissue culture; and   culturing the agricultural substrate to prepare a myceliated agricultural substrate.   
     
     
         2 . The method as set forth in  claim 1  wherein the fungal liquid tissue culture of is prepared by submerged liquid-state culture. 
     
     
         3 . The method set forth in  claim 1 , wherein the method further comprises sterilizing the myceliated agricultural substrate. 
     
     
         4 . The method of  claim 1 , wherein the fungal species is  Morchella hortensis  or golden  Morchella.    
     
     
         5 . The method of  claim 1 , wherein the agricultural substate is selected from the group consisting of cereals, grains, all species of wheat, rye, brown rice, white rice, red rice, gold rice, wild rice, rice, barley, triticale, rice, sorghum, oats, millets, quinoa, buckwheat, fonio, amaranth, teff and durum. 
     
     
         6 - 15 . (canceled)