Allergy antigen and epitope for same

Abstract

The present invention provides novel antigens of an allergy to fish, methods and kits for diagnosing an allergy to fish, pharmaceutical compositions comprising such an antigen, fishes, fish eggs or processed products of such fish or fish egg in which such an antigen is eliminated, fishes that deliver such fish eggs or are born from such fish egg, and a tester for determining the presence or absence of a fish antigen in an object of interest. The present invention also relates to polypeptides comprising an epitope of an antigen, kits, compositions and methods for diagnosing an allergy, comprising such a polypeptide, pharmaceutical compositions comprising such a polypeptide, and raw materials or processed products in which an antigen comprising such a polypeptide is eliminated or reduced. The present invention further relates to a tester for determining the presence or absence of an antigen in an object of interest.

1 . A polypeptide specifically binding to an IgE antibody from an allergic patient, the polypeptide being any one of the following:
 (1α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 150-154 and 205-227;   (2α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 155 and 228-230;   (3α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 156, 157 and 231-237;   (4α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 158, 159 and 238-247;   (5α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 160-162 and 248-261;   (6α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 163-167 and 262-279;   (7α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 168-171 and 280-300;   (8α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 172-174 and 301-310;   (9α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 175-178 and 311-326;   (10α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 179-185 and 327-365;   (11α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 186 and 366-370;   (12α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 187-196 and 371-413;   (13α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 197 and 414-417;   (14α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 198 and 418-420;   (15α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 199 and 421-425;   (16α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 200-202 and 426-436; and   (17α) a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 203, 204 and 437-444.   
     
     
         2 . The polypeptide according to  claim 1 , wherein the number of amino acid residues is 500 or less. 
     
     
         3 . A kit for diagnosing an allergy, comprising at least one of polypeptides according to  claim 1 . 
     
     
         4 . A composition for diagnosing an allergy, the composition comprising at least one of polypeptides according to  claim 1  as an antigen. 
     
     
         5 . A method for providing an indicator for diagnosing an allergy in a subject, the method comprising the steps of:
 (i) contacting a sample obtained from the subject with an antigen, wherein the sample is a solution comprising an IgE antibody;   (ii) detecting binding between the IgE antibody present in the sample obtained from the subject and the antigen; and   (iii) when the binding between the IgE antibody in the subject and the antigen is detected, an indicator of the fact that the subject is allergic is provided;   
       wherein the antigen is at least one of polypeptides according to  claim 1 . 
     
     
         6 . A pharmaceutical composition comprising at least one of polypeptides according to  claim 1 . 
     
     
         7 . The pharmaceutical composition according to  claim 6 , wherein the pharmaceutical composition is intended for the treatment of an allergy. 
     
     
         8 . A tester for determining the presence or absence of an antigen in an object of interest, the tester comprising an antibody that binds to at least one of polypeptides according to  claim 1 . 
     
     
         9 . A tester for determining the presence or absence of an antigen in an object of interest, the tester comprising any of the following primers:
 (a) a primer comprising a portion of the nucleotide sequence of a nucleic acid encoding a polypeptide according to  claim 1 , and/or a portion of a complementary strand thereof; and   (b) a primer which is a portion of at least one of the nucleotide sequences of SEQ ID NOs: 69, 109, 120, 137 and 143 and/or a primer which is a portion of a sequence complementary to at least one of the nucleotide sequences of SEQ ID NOs: 69, 109, 120, 137 and 143.   
     
     
         10 . A raw material or a processed product in which an antigen is eliminated or reduced, wherein the antigen is at least one of polypeptides according to  claim 1 . 
     
     
         11 . A method for producing a processed product in which an antigen is eliminated or reduced, the method comprising the step of confirming that the antigen is eliminated or reduced in a production process of the processed product, wherein the antigen is at least one of polypeptides according to  claim 1 . 
     
     
         12 . A kit for diagnosing an allergy, comprising at least one of polypeptides according to  claim 2 . 
     
     
         13 . A composition for diagnosing an allergy, the composition comprising at least one of polypeptides according to  claim 2  as an antigen. 
     
     
         14 . A method for providing an indicator for diagnosing an allergy in a subject, the method comprising the steps of:
 (i) contacting a sample obtained from the subject with an antigen, wherein the sample is a solution comprising an IgE antibody;   (ii) detecting binding between the IgE antibody present in the sample obtained from the subject and the antigen; and   (iii) when the binding between the IgE antibody in the subject and the antigen is detected, an indicator of the fact that the subject is allergic is provided;   wherein the antigen is at least one of polypeptides according to  claim 2 .   
     
     
         15 . A pharmaceutical composition comprising at least one of polypeptides according to  claim 2 . 
     
     
         16 . The pharmaceutical composition according to  claim 15 , wherein the pharmaceutical composition is intended for the treatment of an allergy. 
     
     
         17 . A tester for determining the presence or absence of an antigen in an object of interest, the tester comprising an antibody that binds to at least one of polypeptides according to  claim 2 . 
     
     
         18 . A tester for determining the presence or absence of an antigen in an object of interest, the tester comprising any of the following primers:
 (a) a primer comprising a portion of the nucleotide sequence of a nucleic acid encoding a polypeptide according to  claim 2 , and/or a portion of a complementary strand thereof; and   (b a primer which is a portion of at least one of the nucleotide sequences of SEQ ID NOs: 69, 109, 120, 137 and 143 and/or a primer which is a portion of a sequence complementary to at least one of the nucleotide sequences of SEQ ID NOs: 69, 109, 120, 137 and 143.   
     
     
         19 . A raw material or a processed product in which an antigen is eliminated or reduced, wherein the antigen is at least one of polypeptides according to  claim 2 . 
     
     
         20 . A method for producing a processed product in which an antigen is eliminated or reduced, the method comprising the step of confirming that the antigen is eliminated or reduced in a production process of the processed product, wherein the antigen is at least one of polypeptides according to  claim 2 .