Enhanced antivirulent for antibiotic-resistant bacteria

Abstract

The teachings provided herein generally relate to enhanced antivirulents that inactivate antibiotic-resistant bacteria without killing them, and the antivirulents are safe for oral administration. More particularly, the systems taught herein include methods of treating a subject having an antibiotic-resistant bacterial infection, comprising administering an effective amount of a antivirulents to a subject having an antibiotic-resistant bacterial infection.

We claim: 
     
         1 . A method of inhibiting the virulence of a pathogen in the gastrointestinal tract of a subject, comprising:
 identifying a pathogen in the gastrointestinal system of a subject;   orally administering an effective amount of a composition to the subject, the composition comprising a combination of:
 an aqueous extract of pomegranate rind to an aqueous extract of green tea leaves in an aqueous concentration of about 600 ppm to about 900 ppm; and, 
 hydrogen peroxide in an aqueous concentration of about 30 ppm to about 1000 ppm; 
   wherein, the combination inhibits the virulence of the pathogen in the subject by inactivating virulence factors in the pathogen without involving antibiotic resistance mechanisms.   
     
     
         2 . The method of  claim 1 , wherein the pathogen is a methicillin resistant  Staphylococcus aureus  (MRSA). 
     
     
         3 . The method of  claim 1 , wherein the pathogen is an antibiotic-resistant  Klebsiella pneumonia.    
     
     
         4 . The method of  claim 1 , wherein the pathogen is an antibiotic-resistant bacteria selected from the group consisting of  Escherichia coli, Salmonella enterica  serotype  typhimurium, Staphylococcus aureus , a methicillin resistant  Staphylococcus aureus  (MRSA),  Pseudomonas aeruginosa, Listeria monocytogenes, Pasteurella multocida, Proteus vulgaris, Klebsiella pneumonia, Bacillus cereus , and  Bordetella bronchiseptica.    
     
     
         5 . The method of  claim 1 , wherein the pathogen is an antibiotic-resistant  Salmonella enterica  serotype  typhimurium.    
     
     
         6 . The method of  claim 1 , wherein the pathogen is an antibiotic-resistant  Pseudomonas aeruginosa.    
     
     
         7 . A method of treating a subject having an antibiotic-resistant bacterial infection, comprising:
 identifying an antibiotic-resistant bacterial infection in the subject;   orally administering an effective amount of a composition to the subject having the antibiotic-resistant bacterial infection, the composition comprising a combination of:
 an aqueous extract of pomegranate rind to an aqueous extract of green tea leaves in an aqueous concentration of about 600 ppm to about 900 ppm; and, 
 hydrogen peroxide in an aqueous concentration of about 30 ppm to about 1000 ppm; 
   wherein, the administering inhibits the virulence of the antibiotic resistant bacteria in the subject by inactivating virulence factors in the antibiotic-resistant pathogen without involving antibiotic resistance mechanisms.   
     
     
         8 . The method of claim  16 , wherein the antibiotic-resistant bacteria is a methicillin resistant  Staphylococcus aureus  (MRSA). 
     
     
         9 . The method of claim  16 , wherein the antibiotic-resistant bacteria is an antibiotic-resistant  Klebsiella pneumonia.    
     
     
         10 . The method of claim  16 , wherein the antibiotic-resistant bacteria is an antibiotic-resistant bacteria selected from the group consisting of  Escherichia coli, Salmonella enterica  serotype  typhimurium, Staphylococcus aureus , a methicillin resistant  Staphylococcus aureus  (MRSA),  Pseudomonas aeruginosa, Listeria monocytogenes, Pasteurella multocida, Proteus vulgaris, Klebsiella pneumonia, Bacillus cereus , and  Bordetella bronchiseptica.    
     
     
         11 . The method of claim  16 , wherein the antibiotic-resistant bacteria is an antibiotic-resistant  Salmonella enterica  serotype  typhimurium.    
     
     
         12 . The method of claim  16 , wherein the antibiotic-resistant bacteria is an antibiotic-resistant  Pseudomonas aeruginosa.