Clozapine Immunoassay

Abstract

Novel conjugates and immunogens derived from clozapine and antibodies generated by these immunogens are useful in immunoassays for the quantification and monitoring of clozapine in biological fluids.

1 - 10 . (canceled) 
     
     
         11 . An antibody which binds selectively to clozapine and does not substantially bind to N-desmethylclozapine and clozapine-N-oxide. 
     
     
         12 . The antibody of  claim 11 , wherein said antibody is derived from mice, sheep, rabbits or rats. 
     
     
         13 . The antibody of  claim 11 , wherein said antibody is a monoclonal antibody. 
     
     
         14 . The antibody of  claim 11 , wherein said antibody is derived from an immunogenic carrier having a reactive amino or thiol group polymer conjugated to a compound selected from the group consisting of compounds of the formula: 
       
         
           
           
               
               
           
         
         wherein B is: —(C═O)—, —(C═O)—NH—, —(C═O)—O—, —CH2-; 
         Y is an organic spacing group; 
         X is a terminal functional group capable of binding 
         to a polyamine polymer; and 
         p is an integer from 0 to 1; 
         or a thereof. 
       
     
     
         15 . The antibody of  claim 14 , wherein the carrier contains a thiol group and X in the compound which is conjugated to the immunogenic polymer is a functional group capable of reacting with said thiol. 
     
     
         16 . The antibody of  claim 15 , wherein X in said compound is 
       
         
           
           
               
               
           
         
       
     
     
         17 . The antibody of  claim 16 , wherein Y in said compound is lower alkylene. 
     
     
         18 . The antibody of  claim 17 , wherein the immunogenic carrier contains as the functional group: 
       
         
           
           
               
               
           
         
       
       wherein v is an integer from 1 to 6. 
     
     
         19 . The antibody of  claim 18 , wherein said antibody is derived from mice, sheep, rabbits or rats. 
     
     
         20 - 38 . (canceled)