Collagen 7 compositions and methods of using the same

Abstract

The present disclosure relates to compositions comprising collagen 7 and methods of using the same to treat DEB.

1 . A composition comprising at least 0.5 mg/ml recombinant collagen 7 (rCo17) in a formulation comprising arginine. 
     
     
         2 . The composition of  claim 1 , wherein rCol7 is at a concentration of about 1.0 mg/ml to about 4.0 mg/ml. 
     
     
         3 . The composition of  claim 2 , wherein rCol7 is at a concentration of about 1.0 mg/ml to about 2.0 mg/ml. 
     
     
         4 . The composition of  claim 3 , wherein the formulation further comprises 10 mM sodium phosphate, 5 mM sodium citrate, 70 mM sodium chloride, 100 mM arginine, 50 mM sucrose and 0.05% (v/v) polysorbate 20. 
     
     
         5 . The composition of  claim 4  for use of medicine administered to a subject in need through infusion wherein the infusion lasts at least 60 minutes. 
     
     
         6 . A method for treating dystrophic epidermolysis bullosa comprising administering to a subject in need thereof an effective amount of a composition that comprises about 1.0 mg/ml to about 4.0 mg/ml recombinant collagen 7. 
     
     
         7 . The method of  claim 6  wherein the composition is administrated to the subject in need by intravenous administration. 
     
     
         8 . A method for increasing closure between epidermis and dermis of the wounded skin in a subject comprising administering to the subject a composition comprising a recombinant collagen 7 in an arginine containing formulation. 
     
     
         9 . The method of  claim 8  wherein the composition is administrated to the subject in need by intravenous administration. 
     
     
         10 . The method of  claim 6  wherein the composition comprises about 1.0 mg/ml to about 2.0 mg/ml recombinant collagen 7. 
     
     
         11 . The method of  claim 10  wherein the composition further comprises 10 mM sodium phosphate, 5 mM sodium citrate, 70 mM sodium chloride, 100 mM arginine, 50 mM sucrose and 0.05% (v/v) polysorbate 20.