Branched Discrete PEG Constructs

Abstract

Disclosed are general and “substantially pure” branched discrete polyethylene glycol constructs useful in attaching to a variety of biologically active groups, for example, preferential locators, as well as biologics like enzymes, for use in diagnostics, e.g. imaging, therapeutics, theranostics, and moieties specific for other applications. In its simplest intermediate state, a branched branched discrete polyethylene glycol construct is terminated at one end by a chemically reactive moiety, “A”, a group that is reactive with a biologic material that creates “A”, which is a biologically reactive group, connected through to a branched core (BC) which has attached at least two dPEG-containing chains, indicated by the solid line, , having terminal groups, which can be charged, non-reactive or reactable moieties and containing between about 2 and 64 dPEG residues.

1 - 22 . (canceled) 
     
     
         23 . A method of externally imaging an antibody, antibody fragment, or a peptide attached to an externally imagable radiolabel, which comprises attaching said imagable radiolabel to and said antibody, antibody fragment, or a peptide to the structure of claim  10 , wherein RG is said radiolabel and A comprises said antibody, antibody fragment, or a peptide. 
     
     
         24 . The method of  claim 23 , wherein said antibody has been generated against a tumor associated glycoprotein. 
     
     
         25 . The method of  claim 24 , wherein said antibody comprises B72.3, CC49, V59, or 3E8. 
     
     
         26 . The method of  claim 25 , wherein said antibody comprises CC49, domain deleted CC49, CC49 fragments, including Fab′, CC49 diabodies, CC49 scFv, and single chains derived therefrom. 
     
     
         27 . The method of  claim 23 , wherein said antibody comprises 3E8, domain deleted 3E8, 3E8 fragments, 3E8 diabodies, and single chains derived therefrom. 
     
     
         28 - 31 . (canceled)