Method for stabilizing extracellular vesicles

Abstract

The present invention provides stabilization of an extracellular vesicle. Specifically, the present invention provides, for example, a method of stabilizing the extracellular vesicle including mixing an extracellular vesicle-containing sample with a saccharide and a chelating agent.

1 . A method of stabilizing an extracellular vesicle, the method comprising mixing an extracellular vesicle-containing sample with a saccharide and a chelating agent. 
     
     
         2 . The method of  claim 1 , wherein the extracellular vesicle is an exosome. 
     
     
         3 . The method of  claim 1 , wherein the extracellular vesicle-containing sample is a body fluid or a culture supernatant. 
     
     
         4 . The method of  claim 1 , wherein the extracellular vesicle-containing sample is a blood sample. 
     
     
         5 . The method of  claim 1 , wherein a concentration of the sacchande in the mixing is 2.5 to 100 mg/mL. 
     
     
         6 . The method of  claim 1 , wherein a concentration of the chelating agent in the mixing is 1 to 200 mM. 
     
     
         7 . A method of stabilizing an extracellular vesicle, the method comprising:
 (1) mixing an extracellular vesicle-containing sample with a saccharide, to obtain a mixture; and   (2) freezing the mixture.   
     
     
         8 . The method of  claim 7 , wherein the freezing comprises freeze-drying. 
     
     
         9 . A method of stabilizing an extracellular vesicle, the method comprising:
 (1) mixing an extracellular vesicle-containing sample with a chelating agent, to obtain a mixture; and   (2) freezing the mixture.   
     
     
         10 . The method of  claim 9 , wherein the freezing comprises freeze-drying. 
     
     
         11 - 12 . (canceled) 
     
     
         13 . A stabilized extracellular vesicle obtained by the method of  claim 1 . 
     
     
         14 . A stabilized extracellular vesicle obtained by the method of  claim 7 . 
     
     
         15 . A stabilized extracellular vesicle obtained by the method of  claim 9 .