Soluble B7-H1

Abstract

This document features methods of evaluating mammals by assessing expression of B7-H1 in a body fluid.

1 - 20 . (canceled) 
     
     
         21 . A method for preparing a solid substrate bound by soluble B7-H1, said method comprising contacting (a) a sample of body fluid from a mammal with (b) a solid substrate having capture antibodies attached thereto, wherein the capture antibodies have binding affinity for soluble B7-H1, and wherein the contacting is under conditions in which soluble B7-H1 in said sample binds to said solid substrate to form a reacted solid substrate. 
     
     
         22 . The method of  claim 21 , further comprising washing said reacted solid substrate. 
     
     
         23 . The method of  claim 21 , wherein said soluble B7-H1 is sB7-H1 Δ531-636 . 
     
     
         24 . The method of  claim 21 , wherein said mammal is a human. 
     
     
         25 . The method of  claim 21 , wherein said body fluid is selected from the group consisting of blood, plasma, serum, and urine. 
     
     
         26 . The method of  claim 21 , wherein said mammal is suspected of having a cancer. 
     
     
         27 . The method of  claim 26 , wherein said cancer is renal cell carcinoma. 
     
     
         28 . The method of  claim 21 , further comprising contacting said reacted solid substrate with a reporter antibody having binding affinity for soluble B7-H1. 
     
     
         29 . The method of  claim 28 , wherein said reporter antibody comprises a label selected from the group consisting of a radioisotope, a fluorophore, a luminescent moiety, biotin, and an enzyme. 
     
     
         30 . The method of  claim 21 , wherein said solid substrate is a bead or a microtiter plate.