Peptide and method for reducing the phosphate requirement and excretion from farm animals

Abstract

Disclosed are methods of altering metabolism of phosphate in a livestock via an antibody. The methods involve the development of an antibody against FGF-23 for the purpose of reducing phosphate excretion by an animal.

What is claimed is: 
     
         1 . An FGF-23 epitope polypeptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:16, SEQ ID NO: 17, and SEQ ID NO:18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22 and SEQ ID NO: 23, wherein the FGF-23 epitope polypeptide is conjugated to a carrier protein. 
     
     
         2 . The FGF-23 epitope polypeptide of  claim 1 , wherein the carrier protein is selected from the group consisting of bovine gamma globulin, FGF-23, bovine serum albumin, keyhole limpet hemocyanin, and ovalbumin. 
     
     
         3 . A vaccine composition comprising the FGF-23 epitope polypeptide of  claim 1 , wherein the vaccine composition is an injectable composition comprising an immunological adjuvant. 
     
     
         4 . The vaccine composition of  claim 3 , wherein the carrier protein is selected from the group consisting of bovine gamma globulin, FGF-23, bovine serum albumin, keyhole limpet hemocyanin, and ovalbumin. 
     
     
         5 . A method of eliciting an immune response in a livestock comprising introducing into the livestock an FGF-23 epitope polypeptide consisting of an amino acid sequence selected from the group consisting SEQ ID NO:16, SEQ ID NO: 17, and SEQ ID NO:18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22 and SEQ ID NO: 23, wherein the FGF-23 epitope polypeptide is conjugated to a carrier protein. 
     
     
         6 . The method of  claim 6 , wherein the livestock is an avian. 
     
     
         7 . The method of  claim 6 , wherein the livestock is a chicken, a turkey, a duck, a pheasant, a quail, a fish, a sheep, a swine, or a cow. 
     
     
         8 . The method of  claim 6 , wherein introducing comprises injecting the livestock with the FGF-23 epitope polypeptide. 
     
     
         9 . A method of reducing dietary phosphate requirements of livestock and livestock offspring, the method comprising
 inducing production of a maternal antibody by introducing the FGF-23 epitope polypeptide of  claim 1  into a fertile livestock female, and   fertilizing at least one egg of the livestock, wherein said maternal antibody is transferred to the livestock offspring via egg yolk, placenta or colostrum, thereby altering the livestock offspring's metabolism of the dietary nutrient.   
     
     
         10 . The method of  claim 9 , wherein the dietary nutrient is phosphate. 
     
     
         11 . The method of  claim 9 , wherein the conjugated vaccine is introduced into the livestock in an amount between about 50 micrograms and about 6 milligrams. 
     
     
         12 . A method of altering phosphate metabolism in a livestock, the method comprising:
 inducing production of an antibody by introducing the FGF-23 epitope polypeptide of  claim 1  into the livestock.   
     
     
         13 . The method of  claim 12 , wherein production of the antibody is induced by injecting the livestock with the FGF-23 epitope polypeptide. 
     
     
         14 . The method of  claim 12 , further comprising feeding the livestock a diet comprising citric acid.