Methods and systems for analyzing image data

Abstract

Methods and systems for analysis of image data generated from various reference points. Particularly, the methods and systems provided are useful for real time analysis of image and sequence data generated during DNA sequencing methodologies.

What is claimed is: 
     
         1 . A method of generating a phasing-corrected intensity value comprising:
 (a) performing a plurality of cycles of a sequencing by synthesis reaction such that, at each cycle, a signal is generated indicative of incorporation of the same nucleotide into a plurality of identical polynucleotides, whereby a portion of the signal is noise associated with a nucleotide incorporated during a previous cycle;   (b) detecting the signal at each cycle, the signal having an intensity value; and   (c) correcting the intensity value for phasing by applying a first order phasing correction to the intensity value;   wherein a new first order phasing correction is calculated for each cycle.