Expression and secretion system

Abstract

The invention provides an expression and secretion system, and methods of using the same, for the expression and secretion of one fusion protein in prokaryotic cells and a second fusion protein in eukaryotic cells. Also provided herein are nucleic acid molecules, vectors and host cells comprising such vectors and nucleic acid molecules.

1 - 16 . (canceled) 
     
     
         17 . A polypeptide comprising, in an N-to-C terminal orientation:
 (i) a signal peptide comprising the amino acid sequence of SEQ ID NO: 3;   (ii) a variable domain, wherein the variable domain comprises an HVR1, an HVR2, and an HVR3; and   (iii) a coat protein or adaptor protein.   
     
     
         18 . The polypeptide of  claim 17 , wherein the variable domain is a variable heavy chain (VH) domain. 
     
     
         19 . The polypeptide of  claim 18 , wherein the VH domain is N-terminal to a CH1 domain, and wherein the CH1 domain is N-terminal to the coat protein or adaptor protein. 
     
     
         20 . The polypeptide of  claim 18 , wherein the polypeptide further comprises a variable light chain (VL) domain which is N-terminal to the VH domain. 
     
     
         21 . The polypeptide of  claim 20 , wherein the VL domain is N-terminal to a CL domain, and wherein the CL domain is N-terminal to the VH domain. 
     
     
         22 . The polypeptide of  claim 17 , wherein the variable domain is a VL domain. 
     
     
         23 . The polypeptide of  claim 22 , wherein the VL domain is N-terminal to a CL domain. 
     
     
         24 . The polypeptide of  claim 23 , wherein the CL domain is N-terminal to the coat protein or adaptor protein. 
     
     
         25 . The polypeptide of  claim 22 , wherein the polypeptide further comprises a VH domain which is N-terminal to the VL domain. 
     
     
         26 . The polypeptide of  claim 25 , wherein the VH domain is N-terminal to a CH1 domain, and wherein the CH1 domain is N-terminal to the VL domain. 
     
     
         27 . The polypeptide of  claim 17 , wherein the variable domain is linked to a utility peptide. 
     
     
         28 . The polypeptide of  claim 27 , wherein the utility peptide is selected from the group consisting of a control protein, a tag, or a label. 
     
     
         29 . The polypeptide of  claim 28 , wherein the control protein is a gD protein, or a fragment thereof. 
     
     
         30 . The polypeptide of  claim 17 , wherein the coat protein is pI, pII, pIII, pIV, pV, pVI, pVII, pVIII, pIX, and pX of bacteriophage M13, f1, and fd. 
     
     
         31 . The polypeptide of  claim 30 , wherein the coat protein is amino acids 267-421 or 262-418 of the pIII protein. 
     
     
         32 . A polypeptide comprising, in an N-to-C terminal orientation:
 (i) a signal peptide comprising the amino acid sequence of SEQ ID NO: 3, wherein the signal peptide is functional in both a prokaryotic cell and a eukaryotic cell;   (ii) a VH domain comprising a VH-HVR1, a VH-HVR2, and a VH-VHR3; and   (iii) an Fc region.   
     
     
         33 . The polypeptide of  claim 32 , wherein the polypeptide further comprises a VL domain which is N-terminal to the VH domain. 
     
     
         34 . The polypeptide of  claim 33 , wherein the polypeptide is an antibody or antibody fragment. 
     
     
         35 . An antibody comprising, in an N-to-C terminal orientation:
 (i) a first signal peptide, wherein the first signal peptide is functional in both a prokaryotic cell and a eukaryotic cell;   (ii) a VL domain;   (iii) a second signal peptide, wherein the second signal peptide comprises the amino acid sequence of SEQ ID NO: 3;   (iv) a VH domain; and   (v) an Fc region.