Herbal composition for inhibiting melanoma cell metastasis and manufacturing method thereof

Abstract

The present invention relates to a Chinese herbal medicine composition which is prepared from a raw material comprising the following ratio: 1 part by weight of Lilium brownii var viridulum (Lily Bulb), 5 parts by weight of Houttuynia cordata (Heartleaf Houttuynia Herb), 2 parts by weight of Atractylodes macrocephala (Rhizome), 2 parts by weight of Poria cocos (Schw) Wolf (Tuckahoe), 1 part by weight of Ziziphi Spinosae Semen (Spine Date Seed), 3 parts by weight of Adenophora morrisonensis (American silvertop root), 2 parts by weight of Semen Astragali Complanati (Flatstem Milkvetch Seed), 0.5 parts by weight of Zingiber officinale (Fresh Ginger), 5 parts by weight of Tribulus terrestris (Puncturevine Caltrap Fruit), 3 parts by weight of Pogostemon cablin (Cablin Potchouli Herb), 0.5 parts by weight of Ziziphus jujube (Common Jujube), and 0.1 parts by weight of dried Dimocarpus longan (Dried Longan Pulp). The invention also relates to a method of using the herbal composition for inhibiting melanoma cell metastasis. The invention also relates to a process for the preparation of the herbal composition.

1 . A Chinese herbal medicine composition for inhibiting melanoma cell metastasis which is prepared from a raw material comprising the following ratio: 1 part by weight of  Lilium brownii  var.  viridulum,  5 parts by weight of  Houttuynia cordata,  2 parts by weight of  Atractylodes macrocephala,  2 parts by weight of  Poria cocos  (Schw.) Wolf, 1 part by weight of  Ziziphi Spinosae Semen,  3 parts by weight of  Adenophora morrisonensis,  2 parts by weight of  Semen Astragali Complanati,  0.5 parts by weight of  Zingiber officinale,  5 parts by weight of  Tribulus terrestris,  3 parts by weight of  Pogostemon cablin,  0.5 parts by weight of  Ziziphus jujube , and 0.1 parts by weight of  Dimocarpus longan.    
     
     
         2 . The Chinese herbal medicine composition of  claim 1 , wherein the  Tribulus terrestris  is  Tribulus taiwanese  belonging to the Zygophyllaceae family. 
     
     
         3 . A method for inhibiting melanoma cells metastasis by administering to a subject in need thereof a reasonable dose of the Chinese herbal medicine composition of  claim 1  to achieve an effect of inhibiting melanoma metastasis. 
     
     
         4 . The method of  claim 3 , wherein the melanoma cells metastasis is metastasis to lungs. 
     
     
         5 . The method of  claim 3 , wherein the reasonable dose is from 1 to 5 g of the Chinese herbal medicine composition per kg body weight of the subject in need thereof. 
     
     
         6 . The method of  claim 3 , wherein the Chinese herbal medicine composition is administered orally to the subject in need thereof. 
     
     
         7 . The method of  claim 3 , wherein the Chinese herbal medicine composition is administered to the subject in need thereof for 14 consecutive days. 
     
     
         8 . A method for preparing a Chinese herbal medicine composition of  claim 1 , which comprises the following steps of:
 soaking 5 parts by weight of  Tribulus terestris  and 5 parts by weight of  Houttuvnia cordata  in an organic solvent and filtering to form a filtrate;   concentrating the filtrate into an extract;   soaking 1 part by weight of  Lilium brownii  var.  viridulum,  2 parts by weight of  Atractylodes macrocephala,  2 parts by weight of  Poria cocos  (Schw.) Wolf, 1 part by weight of  Ziziphi Spinosae Semen,  3 parts by weight of  Adenophora morrisonensis,  2 parts by weight of  Semen Astragali Complanati,  0.5 parts by weight of  Zingiber officinale,  3 parts by weight of  Pogostemon cablin,  0.5 part by weight of  Ziziphus jujube  and 0.1 part by weight of dried  Dimocarpus longan  in water to obtain a soaking liquid;   concentrating the soaking liquid to obtain a concentrated liquid;   and mixing the extract and the concentrated liquid, subjecting to high temperature and high pressure, and concentrating to obtain the Chinese herbal medicine composition.   
     
     
         9 . The method of  claim 8 , wherein the organic solvent is ethanol. 
     
     
         10 . The method of  claim 9 , wherein the concentration of ethanol is 20%. 
     
     
         11 . The method of  claim 8 , wherein the concentration of the filtrate is increased by 7 fold to obtain the extract, 
     
     
         12 . The method of  claim 8 , wherein the concentration of the soaking liquid is increased by 10 fold to obtain the concentrated liquid.