Disposable cell culture bag

Abstract

Disclosed is a disposable cell culture bag capable of facilitating cell culture by using a flexible transparent resin film, the disposable cell culture bag including a body having a sealed space for accommodating a medium, and made of a flexible transparent resin film usable for disposable products, and one or more tubing lines extending from an inside to an outside of the body, wherein the body includes a bottom-side integrated part provided by extending one or more side surfaces from a bottom surface of the body to be spread in a radial shape, in order to obtain continuously smooth four edges of the bottom surface, and a top surface fusion bonded to the side surfaces of the bottom-side integrated part after the side surfaces are fusion bonded to each other.

1 . A disposable cell culture bag comprising:
 a body having a sealed space for accommodating a medium, and made of a flexible transparent resin film usable for disposable products; and   one or more tubing lines extending from an inside to an outside of the body,   wherein the body comprises:   a bottom-side integrated part provided by extending one or more side surfaces from a bottom surface of the body to be spread in a radial shape, in order to obtain continuously smooth four edges of the bottom surface; and   a top surface fusion bonded to the side surfaces of the bottom-side integrated part after the side surfaces are fusion bonded to each other.   
     
     
         2 . The disposable cell culture bag of  claim 1 , wherein the bottom-side integrated part comprises first fusion bonding lines at boundaries and is provided in a cross shape by extending four side surfaces from the bottom surface. 
     
     
         3 . The disposable cell culture bag of  claim 1 , wherein the top surface has a rectangular shape corresponding to the side surfaces of the bottom-side integrated part, and comprises second fusion bonding lines at boundaries. 
     
     
         4 . The disposable cell culture bag of  claim 1 , wherein each of the tubing lines comprises:
 a tube penetrating through the body; and   a flange protruding in an outer circumferential direction of the tube to fix the tube to the body, and fusion bonded to an internal surface of the body.   
     
     
         5 . The disposable cell culture bag of  claim 1 , wherein the tubing lines comprise one or more of a temperature sensor line, a sampling line, a feeding line, a gas-in line, a gas-out line, an air line, a base line, and a combination thereof. 
     
     
         6 . The disposable cell culture bag of  claim 5 , wherein ends of the tubing lines are sealed with fasteners to prevent external air from entering while air is tightly filled in the body. 
     
     
         7 . The disposable cell culture bag of  claim 1 , wherein the tubing lines extend along the side surfaces of the body to the bottom surface to minimize bubbles formed when a gas or a liquid is supplied from outside to the medium. 
     
     
         8 . The disposable cell culture bag of  claim 1 , wherein a patch for measuring an acid level and a patch for measuring an oxygen saturation level are provided in the body. 
     
     
         9 . The disposable cell culture bag of  claim 1 , wherein fusion bonded wings are provided outside the body.