Method of separating vesicle from sample

Abstract

A method of separating a vesicle from a sample may include forming a fusant by fusing a first lipid membrane structure and the vesicle, where the vesicle may include a lipid bilayer membrane, and where the first lipid membrane structure may include a vesicular membrane-fusogenic lipid, and fusing the fusant and a second lipid membrane structure positioned at a predetermined field on a substrate.

What is claimed is: 
     
         1 . A method of separating a vesicle from a sample, comprising:
 forming a fusant by fusing a first lipid membrane structure and the vesicle, the vesicle comprising a lipid bilayer membrane, and the first lipid membrane structure comprising a vesicular membrane-fusogenic lipid; and   fusing the fusant and a second lipid membrane structure positioned at a predetermined field on a substrate.   
     
     
         2 . The method of separating the vesicle from a sample according to  claim 1 , further comprising:
 attracting the fusant to the predetermined field, wherein   the first lipid membrane structure comprises a characteristic-imparting substance encapsulated in the vesicular membrane-fusogenic lipid.   
     
     
         3 . The method of separating the vesicle from a sample according to  claim 2 , wherein the attracting is performed by generating a magnetic force. 
     
     
         4 . The method of separating the vesicle from a sample according to  claim 1 , wherein the predetermined field is a recess portion on the substrate. 
     
     
         5 . The method of separating the vesicle from a sample according to  claim 1 , further comprising:
 causing a reaction between a reaction reagent in the predetermined field and a constituent of the vesicle after fusing the fusant and the second lipid membrane structure.   
     
     
         6 . The method of separating the vesicle from a sample according to  claim 5 , further comprising:
 detecting a reaction product of the reaction.   
     
     
         7 . The method of separating the vesicle from a sample according to  claim 1 , wherein the first lipid membrane structure comprises a membrane fusion-inducing substance. 
     
     
         8 . A method of separating a vesicle from a sample, comprising:
 forming a fusant by fusing a first lipid membrane structure and the vesicle, the vesicle comprising a lipid bilayer membrane, and the first lipid membrane structure comprising a vesicular membrane-fusogenic lipid; and   fusing the fusant and a second lipid membrane structure which closes an opening of a recess portion on a substrate.   
     
     
         9 . The method of separating the vesicle from a sample according to  claim 8 , further comprising:
 attracting the fusant to the recess portion, wherein   the first lipid membrane structure comprises a characteristic-imparting substance encapsulated in the vesicular membrane-fusogenic lipid.   
     
     
         10 . The method of separating the vesicle from a sample according to  claim 9 , wherein the attracting is performed by generating a magnetic force. 
     
     
         11 . The method of separating the vesicle from a sample according to  claim 8 , further comprising:
 causing a reaction between a reaction reagent in the recess portion and a constituent of the vesicle after fusing the fusant and the second lipid membrane structure.   
     
     
         12 . The method of separating the vesicle from a sample according to  claim 11 , further comprising:
 detecting a reaction product of the reaction.   
     
     
         13 . The method of separating the vesicle from a sample according to  claim 8 , wherein the first lipid membrane structure comprises a membrane fusion-inducing substance. 
     
     
         14 . A method of separating a vesicle from a sample, comprising:
 forming a fusant by fusing a first lipid membrane structure and the vesicle, the vesicle comprising a lipid bilayer membrane, and the first lipid membrane structure comprising a vesicular membrane-fusogenic lipid; and   fusing the fusant and a second lipid membrane structure positioned at a predetermined field on a substrate, the second lipid membrane structure comprising a vesicular membrane-fusogenic lipid.   
     
     
         15 . The method of separating the vesicle from a sample according to  claim 14 , further comprising:
 attracting the fusant to the predetermined field, wherein   the first lipid membrane structure comprises a characteristic-imparting substance encapsulated in the vesicular membrane-fusogenic lipid.   
     
     
         16 . The method of separating the vesicle from a sample according to  claim 14 , wherein the attracting is performed by generating a magnetic force. 
     
     
         17 . The method of separating the vesicle from a sample according to  claim 14 , wherein the predetermined field is a recess portion on the substrate. 
     
     
         18 . The method of separating the vesicle from a sample according to  claim 14 , further comprising:
 causing a reaction between a reaction reagent in the second lipid membrane structure and a constituent of the vesicle after fusing the fusant and the second lipid membrane structure.   
     
     
         19 . The method of separating the vesicle from a sample according to  claim 18 , further comprising:
 detecting a reaction product of the reaction.   
     
     
         20 . The method of separating the vesicle from a sample according to  claim 14 , wherein the first lipid membrane structure comprises a membrane fusion-inducing substance.