Gold and palmitoyl pentapeptide-4 nanoparticles

Abstract

The present invention relates to gold nanoparticles conjugated with palmitoyl pentapeptide-4 (Palm-L-Lys-L-Thr-L-Thr-L-Lys-L-Ser-OH), compositions comprising them, use thereof in cosmetic treatments, as well as methods for obtaining said nanoparticles.

1 . Gold nanoparticles conjugated with a peptide of formula (I): 
       
         
           
                 
                 
               
                     
                   (I) 
                 
                     
                   (R-SEQ ID NO: 1) 
                 
                     
                   R-L-Lys-L-Thr-L-Thr-L-Lys-L-Ser-OH 
                 
             
                
                
                
               
            
           
         
       
       wherein R is a C 10 -C 22  acyl moiety. 
     
     
         2 . The nanoparticles according to  claim 1 , wherein the R moiety is a palmitoyl moiety. 
     
     
         3 . The nanoparticles according to  claim 1 , wherein the nanoparticles mean diameter is less than 500 nm. 
     
     
         4 . The nanoparticles according to  claim 3 , wherein the nanoparticles mean diameter is comprised between 50 and 200 nm. 
     
     
         5 . The nanoparticles according to  claim 1 , wherein the nanoparticles exhibit a zeta potential comprised between −5 mV and −60 mV. 
     
     
         6 . The nanoparticles according to  claim 1 , wherein a concentration of the peptide of formula (I) is from 0.01 to 1 mg of peptide (I) per mg of nanoparticles. 
     
     
         7 . A composition comprising the nanoparticles defined in  claim 1  and water. 
     
     
         8 . The composition according to  claim 7 , wherein the composition contains at least 95% (w/w) of water with respect to a sum of the weight of water and nanoparticles in the composition. 
     
     
         9 . The composition according to  claim 7  further comprising a cosmetic agent selected from the group consisting of surfactants, moisturizing agents, antioxidants, emollients, preservatives, humectants, viscosity modifiers, and mixtures thereof. 
     
     
         10 . The composition according to  claim 7 , wherein the composition is in the form of a cream, serum, emulsion, gel, foam, paste, ointment, milk, or solution. 
     
     
         11 . A process for the preparation of a composition comprising the nanoparticles of  claim 1  comprising using the nanoparticles as defined in  claim 1 . 
     
     
         12 . A process of taking care of the skin comprising applying thereto nanoparticles according to  claim 1  or a composition comprising the nanoparticles according to  claim 1  for skin care. 
     
     
         13 . A process according to  claim 12 , wherein skin care is the cosmetic treatment of wrinkles and/or cosmetic prevention of the onset of wrinkles. 
     
     
         14 . A method for obtaining nanoparticles as defined in  claim 1  comprising:
 (a) reducing Au(III) from a compound containing said Au(III) to Au(0) by means of treatment with a reducing agent to form gold nanoparticles, and 
 (b) treating the gold nanoparticles obtained in step (a) with the peptide of formula (I). 
 
     
     
         15 . The method according to  claim 14 , wherein the reducing agent of step (a) is sodium citrate. 
     
     
         16 . The method according to  claim 14 , wherein steps (a) and (b) are carried out in water. 
     
     
         17 . The method according to  claim 14 , wherein the compound of Au(III) of step (a) is HAuCl 4 .