Bacillus compositions and uses thereof

Abstract

A spore-forming Bacillus species, and more particularly, a Bacillus subtilis strain identified as MB40 is provided. Compositions comprising the MB40 strain, methods of making products comprising the same, and methods of using the same are also provided.

1 - 46 . (canceled) 
     
     
         47 . A method for inhibiting the growth of pathogenic bacteria on a surface comprising applying a composition comprising an effective amount of  Bacillus subtilis  MB40, a sample of which has been deposited under ATCC Accession No. PTA-122264, to the surface. 
     
     
         48 . The method of  claim 47 , wherein the composition further comprises water. 
     
     
         49 . The method of  claim 47 , wherein the composition further comprises one or more of the following: ionic or nonionic surfactants; a disinfectant comprising an antimicrobial or antifungal compound; one or more salts, and/or one or more oxidizing agents. 
     
     
         50 . The method of  claim 47 , wherein the composition comprises a dry powder. 
     
     
         51 . The method of  claim 47 , wherein the  Bacillus subtilis  MB40 is present as live cells and/or spores at a concentration of at least 1×10 6 , 1×10 7 , 1×10 8 , 1×10 9 , or 1×10 10  CFUs/gram. 
     
     
         52 . The method of  claim 47 , wherein the pathogenic bacteria is a species from  Staphylococcus, Enterococcus, Listeria, Salmonella, Streptococcus, Pseudomonas, Campylobacter, Clostridium, Klebsiella, Escherichia, Acinetobacter, Aeromonas, Pasteurella , or  Bordetella.    
     
     
         53 . The method of  claim 52 , wherein the Staphylococcus species is S. aureus, methicillin-resistant  S. aureus , vancomycin-intermediate  S. aureus , vancomycin-resistant  S. aureus  (VRSA), or linezolid-resistant  S. aureus.    
     
     
         54 . The method of  claim 52 , wherein the  Streptococcus  species is  S. pneumonia.    
     
     
         55 . The method of  claim 52 , wherein the  Listeria  species is  L. monocytogenes.    
     
     
         56 . The method of  claim 52 , wherein the  Campylobacter  species is  C. jejuni.    
     
     
         57 . The method of  claim 52 , wherein the  Clostridium  species is  C. difficile  or  C. perfringens    
     
     
         58 . The method of  claim 52 , wherein the  Enterococcus  species is  E. faecalis , vancomycin-resistant  E. faecium  or linezolid-resistant  E. faecium.    
     
     
         59 . The method of  claim 47 , wherein the surface is a hard surface. 
     
     
         60 . The method of  claim 47 , wherein the surface is a porous surface. 
     
     
         61 . The method of  claim 47 , wherein the surface is a rug, sink, faucet, toilet, or drain.