Method for manufacturing stem cell having appropriate size for intravascular administration

Abstract

The present invention relates to a method for preparing stem cells having a size suitable for intravascular administration, and preferably to a method for preparing stem cells with a diameter ranging from 11-16 μm. Additionally, the present invention relates to media composition for preparing stem cells having a size suitable for intravascular administration. According to the present invention, stem cells having a size suitable for intravascular administration can be prepared such that stem cells administered into a vein can stably reach a target tissue, and thus can more efficiently increase efficacy displaying activity, thereby innovatively enhancing the efficacy of cell therapy using stem cells.

1 .- 17 . (canceled) 
     
     
         18 . A medium composition for preparing stem cells having a size suitable for intravascular administration, comprising K-SFM containing N-acetyl-L-cysteine (NAC), insulin or insulin-like factor, hydrocortisone, basic fibroblast growth factor (bFGF), about 5% FBS (fetal bovine serum), calcium, EGF (epidermal growth factor) and about 1 ng/ml of selenium. 
     
     
         19 . The medium composition of  claim 18 , wherein the stem cells are adult stem cells. 
     
     
         20 . The method composition of  claim 19 , wherein the stem cells are adipose tissue-derived mesenchymal stem cells.