Multi-Dimensional Biomaterial and Method for Producing the Same

Abstract

Biomaterial having a multi-dimensional structure and comprising demineralized bone matrix dispersed within differentiated mesenchymal stem cells (MSCs) tissue, wherein MSCs are adipose tissue-derived stem cells. Method for producing this biomaterial comprising incubating MSCS in osteoblastic and/or chondrogenic medium in presence of demineralized bone matrix. Use of this biomaterial for alleviating or treating a bone or cartilage defect, supporting or correcting a congenital or acquired abnormality, supporting a bon or articular bone replacement following surgery or trauma, and/or supporting a musculoskeletal implant.

1 .- 17 . (canceled) 
     
     
         18 . Method for producing a multi-dimensional biomaterial having a multi-dimensional structure and comprising differentiated mesenchymal stem cells (MSCs) tissue and demineralized bone matrix, wherein said demineralized bone matrix is dispersed within the differentiated MSCs tissue, and wherein said MSCs are adipose tissue-derived stem cells, said method comprising the steps of:
 a. incubating MSCs in osteoblastic and/or chondrogenic medium during 15-25 days,   b. adding demineralized bone matrix in said media, and   c. maintaining incubation for an additional period of 15-30 days.   
     
     
         19 . The method according to  claim 18 , further comprising the step of replacing the media every 2 days during the additional period, without removing the demineralized bone matrix. 
     
     
         20 . The method according to  claim 18 , wherein the MSCs are late-passaged adipose tissue-derived stem cells. 
     
     
         21 . The method according to  claim 18 , wherein the incubation of step c. is maintained for an additional period of 15-25 days. 
     
     
         22 . The method according to  claim 18 , wherein the incubation of step c. is maintained for an additional period of 20 days. 
     
     
         23 . The method according to  claim 18 , wherein the demineralized bone matrix is added in the media in an amount of 1 to 20 mg/ml. 
     
     
         24 . The method according to  claim 18 , wherein the demineralized bone matrix is added in the media in an amount of 1 to 10 mg/ml. 
     
     
         25 . The method according to  claim 18 , wherein the demineralized bone matrix is added in the media in an amount of 5 to 10 mg/ml. 
     
     
         26 . The method according to  claim 18 , wherein the demineralized bone matrix is in the form of particles having a mean diameter of 50-2500 μm. 
     
     
         27 . The method according to  claim 18 , wherein the osteoblastic medium is a standard medium which is supplemented with Human Serum, dexamethasone, sodium ascorbate, sodium dihydrophosphate, penicillin, and streptomycin. 
     
     
         28 . The method according to  claim 18 , wherein the chondrogenic medium is a standard medium which is supplemented with Human Serum, dexamethasone, TGF-B3, L-proline, sodium ascorbate, sodium dihydrophosphate, sodium pyruvate, Insulin-Transferrin-Selenium, penicillin, and streptomycin. 
     
     
         29 . A multi-dimensional biomaterial obtainable by the method according to  claim 18 .