Sensor, detection method, detection system, and detection device

Abstract

In an aspect, a sensor includes a combining portion 240 that combines with a second substance 220 having a molecular weight larger than a molecular weight of a first substance 210. Further, in an aspect, the sensor includes a substrate 10 including a surface on which the combining portion 240 is disposed, the combining portion 240 detecting whether or not the first substance 210 is included in an analyte that has come into contact with both an aptamer 230 including a first combining part 231 for the first substance 210 and a second combining part 232 for the second substance 220 and being combined with either of the first substance 210 and the second substance 220 and the second substance 220.

1 . A sensor, comprising:
 a substrate comprising a combining portion; and   an aptamer combined with the combining portion before a first contact with an analyte that contains a first substance and combined with the first substance after the first contact with the analyte.   
     
     
         2 . The sensor according to  claim 1 , wherein
 the aptamer is dissociated from the combining portion in response to the first contact with the analyte.   
     
     
         3 . The sensor according to  claim 1 , wherein
 a molecular weight of the aptamer is greater than a molecular weight of the first substance.   
     
     
         4 . The sensor according to  claim 1 , wherein
 the aptamer comprises a first combining part that is configured to be combined with the first substance and a second combining part that is configured to be combined with the combining portion.   
     
     
         5 . The sensor according to  claim 4 , wherein
 at least a part of the first combining part is identical to at least a part of the second combining part.   
     
     
         6 . The sensor according to  claim 1 , wherein
 the aptamer preferentially combines with either the first substance or the combining portion.   
     
     
         7 . The sensor according to  claim 1 , wherein
 a first free energy change that is calculated from a dissociation constant of the aptamer and the first substance is less than a second free energy change associated with a combination of the aptamer and the combining portion.   
     
     
         8 . The sensor according to  claim 1 , further comprising:
 a first cover member, the substrate being positioned on an upper surface of the first cover member; and   a second cover member that is bonded to the first cover member, wherein   at least one of the first cover member and the second cover member comprises an inlet, the analyte flows into the inlet, and   a flow channel that extends from the inlet to at least a surface of the substrate is provided between the first cover member and the second cover member.   
     
     
         9 . The sensor according to  claim 8 , wherein
 the flow channel includes a groove portion formed on a surface of at least one of the first cover member and the second cover member.   
     
     
         10 . The sensor according to  claim 9 , wherein
 the upper surface of the first cover member comprises a concave portion accommodating at least a part of the substrate, and   the second cover member comprises the groove portion.   
     
     
         11 . The sensor according to  claim 1 , further comprising:
 a detecting portion that is positioned on a surface of the substrate, the combining portion being positioned in the detection portion; and   a first IDT electrode and a second IDT electrode on the surface of the substrate, the detecting portion being provided between the first IDT electrode and the second IDT electrode.   
     
     
         12 . The sensor according to  claim 11 , further comprising:
 a first bonding member that is bonded to an upper surface of the substrate and constitutes a first oscillation space hermetically-sealed between the first bonding member and the upper surface of the substrate; and   a second bonding member that is bonded to the upper surface of the substrate and constitutes a second oscillation space hermetically-sealed between the second bonding member and the upper surface of the substrate, wherein   the first oscillation space is positioned on the first IDT electrode, and   the second oscillation space is positioned on the second IDT electrode.   
     
     
         13 . A method for detecting a first substance, comprising:
 preparing a substrate comprising a combining portion that is combined with an aptamter;   causing the substrate to contact an analyte comprising a first substance; and   detecting a change in a surface state of the substrate as a result of transferring the aptamer from the combining portion to the first substance.   
     
     
         14 . A detection device, comprising
 a detection control unit that detects whether a first substance is included in an analyte by detecting a change in a surface state of a substrate of the sensor according to  claim 1 .   
     
     
         15 . A detection system, comprising:
 the sensor according to  claim 1 ; and   a detection device that detects whether a first substance is included in an analyte by detecting a change in a surface state of a substrate of the sensor when the analyte contacts the substrate.