Polypeptides having lipase activity and polynucleotides encoding same

Abstract

The present invention relates to isolated polypeptides having lipase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

1 . An isolated polypeptide having lipase activity, selected from the group consisting of:
 (a) a polypeptide having at least 92% sequence identity to the mature polypeptide of SEQ ID NO: 2;   (b) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, or (ii) the full-length complement of (i);   (c) a polypeptide encoded by a polynucleotide having at least 96% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1;   (d) a variant of the polypeptide of (a), (b) or (c) comprising a substitution, deletion, and/or insertion at one or more positions; and   (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has lipase activity.   
     
     
         2 - 3 . (canceled) 
     
     
         4 . A composition comprising the polypeptide of  claim 1 . 
     
     
         5 . The composition of  claim 4 , wherein said composition is more effective in removing lipid stains present at a surface in comparison with an equivalent composition lacking the lipase. 
     
     
         6 . The composition of  claim 4 , further comprising one or more divalent cations, preferably selected from calcium (Ca 2+ ), magnesium (Mg 2+ ) and iron-II (Fe 2+ ). 
     
     
         7 . The composition of  claim 4 , further comprising one or more surfactants selected from the group consisting of an anionic surfactant, a cationic surfactant, a non-ionic surfactant, zwitterionic surfactant. 
     
     
         8 . The composition of  claim 4 , wherein the surfactant comprises one or more surfactants selected from the group consisting of sodium dodecyl benzene sulfonate, sodium hydrogenated cocoate, sodium laureth sulfate, C12-14 pareth-7, C12-15 pareth-7, sodium C12-15 pareth sulfate, and C14-15 pareth-4. 
     
     
         9 . The composition of  claim 4 , formulated at a pH from 8.0 to 11. 
     
     
         10 . The composition of  claim 4 , wherein the composition is selected from the group consisting of a laundry cleaning composition, a dishwashing cleaning composition, a hard-surface cleaning composition and a personal care cleaning composition. 
     
     
         11 . The composition of  claim 4 , wherein the form of the composition is selected from the group consisting of a liquid, a gel, a soap bar, a powder, a granulated solid, a tablet, or any combination thereof. 
     
     
         12 . The composition of  claim 4 , wherein the composition is effective in hydrolyzing a lipid at a temperature of from about 10° C. to 90° C. 
     
     
         13 . The composition of  claim 7 , wherein the composition is more effective in hydrolyzing lipid substrates compared to an equivalent composition comprising  Thermomyces lanuginosus  lipase (Lipolase™) in place of Lipr139 lipase. 
     
     
         14 . The composition of  claim 4 , further comprising an enzyme selected from the group consisting of hemicellulases, peroxidases, proteases, cellulases, xylanases, lipases, phospholipases, esterases, cutinases, pectinases, mannanases, pectate lyases, keratinases, reductases, oxidases, phenoloxidases, lipoxygenases, ligninases, pullulanases, tannases, pentosanases, malanases, ß-glucanases, arabinosidases, hyaluronidase, chondroitinase, laccase, amylases, and mixtures thereof. 
     
     
         15 . The composition of  claim 4 , wherein the stability of the lipase with at least 92% identity to SEQ ID NO: 2 is greater than the stability of the  Thermomyces lanuginosus  lipase in an equivalent composition comprising  Thermomyces lanuginosus  lipase in place of the lipase with at least 92% identity to SEQ ID NO: 2. 
     
     
         16 . (canceled) 
     
     
         17 . A method for cleaning a surface, comprising contacting a lipid stain present on the surface to be cleaned with the composition of  claim 4 . 
     
     
         18 . A method for hydrolyzing a lipid present in a soil and/or a stain on a surface, comprising contacting the soil and/or the stain with the polypeptide of  claim 1 . 
     
     
         19 . An isolated polynucleotide encoding the polypeptide of. 
     
     
         20 . A nucleic acid construct or expression vector comprising the polynucleotide of  claim 19  operably linked to one or more control sequences that direct the production of the polypeptide in an expression host. 
     
     
         21 . A recombinant host cell comprising the polynucleotide of  claim 19  operably linked to one or more control sequences that direct the production of the polypeptide. 
     
     
         22 . (canceled) 
     
     
         23 . A method of producing a polypeptide having lipase activity, comprising:
 (a) cultivating the host cell of  claim 21  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.